Comparative Study Of Rhizosphere Micro-ecological Environments Of Huperzia Serrata And Dryopteris Fuscipes

Huperzine A, a potential medicine for Alzheimer’s disease, is mainly isolate fromHuperzia serrata (Thunb) Trev. Due to low growth rate, rareness of nature resources,and hard to plant or tissue culture, it still have many problem to large-scale cultivationof Huperzia serrata, and production of huperzine A still depends on nature resources. Inrecent years, because of broad market prospects on huperzine A, over-exploitation leadsHuperzia serrata been greatly restricted to sustainable development and utilization.Therefore, it is necessary to protect nature resources and adopt scientific and effectivemeasures.To understanding the particularity of the rhizosphere micro-ecological environmentof Huperzia serrata, solve the fast reproductive and developmental problems ofHuperzia serrata, and provide scientific theory reference and guidance, in this study,Huperzia serrata and its associated plants (Dryopteris fuscipes) from western Hunanhave been used as the experimental material, the micro-ecological environment hadbeen investigated. The results and conclusions showed as follows:(1) The number of microorganisms from the rhizosphere soil of Huperzia serrataand Dryopteris fuscipes was determined by the plate count method respectively, theresult showed that the three kinds of microbe, bacteria, actinomycetes and fungigroups from Huperzia serrata rhizosphere soil was significantly higher than Dryopterisfuscipes(P <0.05). Analysis the16S rDNA or ITS sequences of each microorganismshowed that the rhizosphere soil microbial species and diversity of Huperzia serrata ishigher than the Dryopteris fuscipes.(2)The enzyme activity of soil invertase, urease and alkaline phosphatase fromHuperzia serrata and Dryopteris fuscipes rhizosphere had been analyzed. The resultswere obtained as follow, the enzyme activity of the three the soil enzyme from Huperziaserrata was higher than the Dryopteris fuscipes, but without significant difference.(3) The physical and chemical properties of rhizosphere of Huperzia serrata andDryopteris fuscipes had been investigated, the results show that the rhizosphere soilphysical and chemical properties of the two plants were basically identical. By usingICP measured mineral elements content of two plants, the measured results showed thatthe significant difference of mineral elements in the plant; Compared with two plantmineral elements of biological absorption coefficient, found that the absorptioncoefficient of P, Mg, etc. large element of Huperzia serrata was lower than Dryopterfuscipes, but the trace elements such as Zn, Cu and Se elements, was higher thanDryopteris fuscipes.(4) By using Excel software analysis the rhizosphere micro-ecology and plant partof the biological characteristics of correlation between the two plants, studies had found that the two kinds of plant’s root length and the rhizosphere actinomycetes existsignificant correlation. P and Mg element biological absorption coefficient of Huperziaserrata and Dryopteris fuscipes, between plant height and total biomass exist a certaincorrelation with significant positive correlation respectively. There is significantpositive correlation between Ca element biological absorption coefficient with theurease and alkaline phosphatase and Mn with bacteria had a significant positivecorrelation.