Research Of Growth And Huperzine-A Accumulation And Antioxidase Activity Of Huperzia Serrata Cultured With Various Amino Acids

We used the Huperzia serrata thallus as tissue culture material and added various amino acids?aspartic acid, lysine, tryptophan? in medium with different concentration to study the differences theantioxidant enzyme in Huperzia serrata thallus and examine the relationship between various amino acid and thallus growth,Huperzine-A accumulation Detecting the activity of antioxidant enzyme,the relative growth rate and the accumulation of Huperzine A of the thallus. The results obtained are listed as followings:1.Supplemented with Trp, the proliferation rate of thallus relative fresh weight was relatively high. The highest relative fresh weight of thallus was supplemented with 1mmol�L^-1 Trp. However, compared with the control, supplemented with Asp,the dry weight accumulation was improved by 18.50%²5.75%.2.The results of HPLC shows that the Hup A of thallus which supplemented with Asp,Lys,Trp and the control were 97.239 ?g�g^-1,61.398 ?g�g^-1,70.801 ?g�g^-1,76.254?g�g^-1.Supplemented with Asp, the HupA accumulation was significantly higher than control group, especially 0.5 mmol�L^-1.Supplemented with Lys or Trp, the HupA accumulation was suppressed.3.Examed the activity of SOD at 10 th day, the activity of SOD was stronger than the control expect of the thallus supplemented with 0.25 mmol�L^-1 Asp. Supplemented with 0.5 mmol�L^-1 Asp,the change patterns of SOD activity was similar with the contrast.Supplemented with 0.25 mmol�L^-1 Lys,the activity of SOD was much stronger than the control excrpt at different growth stages, and the change patterns of SOD activity was different with the contrast.The results of peroxidase isozymes shows that:the isozyme band of the thallus supplemented with 0.5 mmol�L^-1 Asp was different with the control, and expression quantity of POD isozyme was increased in the group supplemented with Asp. The isozyme band of the thallus supplemented with1 mmol�L^-1 Trp was different with the control, but expression quantity of POD isozyme was similarily with the control. The CAT activity of the thallus supplemented with 0.5 mmol�L^-1 Asp shows that the change patterns of CAT activitywas similar with the contrast from 50 d to 70 d,and the activity of CAT was stronger than the control on 80 days. Supplemented with 0.5 mmol�L^-1Asp's,the CAT activity was the strongest.In conclusion,supplemented with Asp, the HupA accumulation was significantly higher than control group, especially 0.5 mmol�L^-1,the HupA accumulation was improved by 1.27.Supplemented with 0.5 mmol�L^-1 Asp, expect of 50 d,the change patterns of SOD activity was similar with the contrast.The isozyme band of the thallus supplemented with 0.5 mmol�L^-1 Asp was different with the control, and expression quantity of POD isozyme was increased on 60 d. Both of SOD, CAT, POD maybe involved in the synthesization for Hup A.