Genetic Diversity Analysis And Studies On The Adaptability Of Different Chickpea Genotypes In Lanzhou

Chickpea (Cicer arietinum L.) belongs to the family Leguminosae and the genus Cicerand is widely cultivated in the world. Its seeds, pods, stem and leaves own high quality forfeeding. Chickpea has been grown in China only for a short time and mainly distributed inXinjiang Uygur Autonomous Region. Domestic germplasm are relatively lack.In this study,16chickpea genotypes introduced from Australia and India were used asthe tested material. Their vegetative and reproductive growth characteristics, diseaseresistance, forage and grain quality was studied in Lanzhou region. Besides, their geneticdiversity was analyzed based on the ISSR marker. The aim of this study was to screen thechickpea genotypes with excellent productivity and quality. The results obtained from thisstudy will be the base for breeding and utilization of chickpea in Lanzhou. The main resultswere as follows:1. The optimal ISSR-PCR amplification system (25μl) for chickpea constructed byorthogonal design L16（45）including3mmol/LMg2+,0.2mmol/L d NTP,0.24μmol/L primerand2U Taq DNA polymerase.Cluster analysis showed that16chickpea genotypes could bedivided into5categories at the GS=0.83. Indian genotype ICCV05111(TypeⅠ), Australiagenotype FLIP97-114C (Type Ⅳ) and Jimbour (TypeⅤ) were clustered into a single category,respectively. The Indian genotypes ICCV98818, ICCV98816and ICCV06107clustered intoone class (type Ⅱ). The other10chickpea genotypes were clustered into one class (TypeⅢ).2. Sixteen chickpea genotypes were divided into mid-matured and late-matured typeaccording to their phenological observation, among which, only the genotype Flipper ismid-matured. The genotype Bumper and FLIP97-114C did not adapted to the climatecondition in Lanzhou. The analysis on the growth rate of chickpea showed that, for16genotypes, the whole growth period could be divided into3phases, including slow,high-speed and rapid growth phase.3. Among16genotypes, high hay yields were observed in the genotypes Jimbour,Jimbour#1, Flipper and ICCV98813, ICCV96853and ICCV96852had high seed yield,and the genotypes ICCV98801, ICCV98816, ICCV06107, Jimbour, Howzat and Flipper were resistant to Ascochyta. Two genotypes with high forage nutrition value were Jimbour#1and Flipper, and another2(Yorker and Bumper) had high crude protein in their grains.