Screening Of Aluminum-resistance Maize Inbred Lines, Physiological Detection And Analysis Of Gene Chip

The acidic soil accounted for about35%of the total land area and50%of arable area in the world.. In our country, the acidic soil is widely distributed in about10province of southern China including Guangdong, Guangxi, Hainan, Hunan, Sichuan etc, a total area of2030000square kilometers, close to1/5of the land area. In acid soil, the first inhibited crop growth of root, then water and mineral nutrient absorption is affected, resulting in decreased crop quality and yield, economic benefits serious decline. Maize is an important grain, feed and economic crop. The cultivated area of maize is increasing every year in our country. However, the aluminum toxicity in acid soil limits maize to the great degree. Acturally, aluminum toxicity, the main limit factor which acid soils attribute40%decline of crop. Screening of aluminum-resistance maize inbred lines, detecting the physiology changes of different cultivar and explore the important genes involved in aluminium tolerance would facilate the undatangding the mechanism of aluminium tolerance in maize. Further, these results will help improve the crop yield in acid soil and full utilization of the potential of acid oil.The experiments were carried out to study the following aspects:(1) After screeningof the141inbred lines on the basis of relative root growth (RRG) under aluminium treatment, all the self inbred lines were classified into three categaries:Aluminumsensitive type(RRG<15%), a total of56inbred lines, Mo17�'�B73are Representative inbred lines; aluminum slightsensitive type (15%<RRG<30%), a total of60inbred lines, CA339and5022(B) are Representative inbred lines; aluminum-tolerance tyope(RRG>30%), a total of25inbred lines, HZ85and178are Representative inbred lines.(2) Digital Scanning data and hematoxylin staining analysis further verifies that the classification of maize according RRG value.That are aluminum-sensitive type such as B73and Mo17, aluminum-slight sensitive typeexampled as CA339and5022(B), aluminum-tolerance type178and HZ85.(3) Comparationof the uptake of Evans Blue and contation of H2O2in the root tip of aluminum-sensitive inbred line B73and aluminum-tolerance inbred line178under aluminum treatment.These result suggested that178is more resistant to aluminium toxicity than B73.(4) Gene expression chip analysis classified the variable expressed genes into7categories which including27important genes need to verify their expression level further.(5) The results of real time PCR revealed that expression data of ALXP1、ALXP3、 ALXP4、ALXP5、ALXP19、ALXP20、ALXP21、ALXP24、ALXP26genes may be negative. Alought the expression levels of ALXP2、ALXP9、ALXP13、ALXP15and ALXP17are to some extent different from the results obtained by real time PCR, the tendency is very similar. The expression of ALXP7、ALXP8、ALXP11、ALXP12、 ALXP14、ALXP22、ALXP25genes detected by real time PCR is in good consistent with the data of gene array analysis,Moreover, the annote biological functions of these genes are related to aluminum tolerance directly or indirectly. It suggested further exploring the mechnisims of these genes in participation in the the regulation of aluminium tolerance is significant.