Establishment Of Tissue Culture Breeding System And Design Factory Nursery Scheme Of Lycoris Albiflora And Lycoris Chinensis

In this study，construct the system of propagation rapidly in tissue culture of Lycoris chinensis andLycoris albiflora.On the basic of the research, The technique of tissue culture and rapid propagation ofL.chinensis is optimized. Growing of L.chinensis to subculture enlargement culture is more effectivethan to propagation culture on liquid medium. The application of the liquid medium reduce effectivelytraining costs and simplify the operation process among the entire process of tissue culture. To Providea theoretical basis and technical support for scale transformation and industrial production of tissueculture of Lycori. The main findings were as follows：1.With base plate of bulb is slected as the best explants for tissue culture of L.chinensis andL.albiflora, and the best time for initial culture of L.chinensis is May to July or November to Januarythe following year, the best time of L.albiflora is April to Mid-July or October to Mid-January thefollowing year. The best method of disinfection: surface sterilization of20s by75%alcohol, washing of2-3times with sterile water, and then disinfection of14minutes by0.1%HgCl2, and dipping4-5times.2. Among the initial culture, the highest induction frequency of bud of L.chinensis is obtained onthe medium of MS+6-BA (1.0mg/L)+NAA (0.2mg/L)+sane sugar (30g/L)+agar-aga (5.5g/L)+pH(5.9), induction rate was88.3%on average;and MS+6-BA (1.0mg/L)+NAA (0.1mg/L)+sane sugar(30g/L)+agar-agar (5.5g/L)+pH (5.9), induction rate was96.7%on average is optimal for inducingof bud of L.albiflora. The best conditions of initial culture is dark for about15days, and then light10hours by intensity1000xl in every day, The method save not only costs, but also improve the survivalrate.3.Among the subculture, A liquid medium replace solid medium is applied for the first time, itsaves not only the costs, but also simplify the operation process. The results show that the best liquidmedium for expanding of L.chinensis is MS+6-BA(1.0mg/L)+NAA (0.5mg/L)+white the sugar (40g/L)+pH (5.9) or MS+6-BA (1.0mg/L)+2,4-D (0.5mg/L)+white sugar (40g/L)+pH (5.9), and MS+KT（0.5mg/L）+NAA（0.5mg/L）+white sugar (30g/L)+pH (5.9) is the most ideal medium for rooting.4.The study provide the main technical parameters for tissue culture of Lycoris to industrialization,for example, the medium formula, hormone proportion, proliferation factor, enlargement culture,subculture number and transplanting matrix and preliminary designed, etc, the program that seedballproduction of Lycoris, drawed its specific process flow diagram, estimated the production costs and benefits. And the paper also give the approximate analysis for the matter of needing in the main links ofactual production of Lycoris, these main links contain varieties determination, medium preparation,initial culture, the conditions of culture, liquid medium for subculture, time of subculture, grade of seed,refined seedlings and transplanting, etc.