Research Of Cross-breeding Of Lycoris

We cultured Lycoris hybrid in this study, combining interspecific and intraspecifichybridization among different species, molecular marker technique and in vitro propagationtechnique, which laied the foundation for shortening new varieties breeding period. The resultsshowed that:1. The pollen viability of different ploidy Lycoris and natural hybrids were tested using invitro pollen germination method. The results showed that the pollen germination rate of diploid ishigher than triploid and hybrid Lycoris. The pollen could be stored for7days in room temperature,about60days in4℃, almost half a year in-20℃.2. We designed12hybridized combinations, using diploid Lycoris species as parents, and theresults showed that almost all setting percentages were above50%, but the affinity rates wereunder40%for all combinations. The results of cross compatibility between L. chinensis and L.radiata showed that erratic deposition of callose in the papilla, pollen tube, canal cell, andgerminated pollen grains on the stigma were less, which were the main causes of cross-incompatibility.3. Germplasm identification of molecular markersAn optimal SCoT-PCR amplification system of Lycoris Herb.was established:2.0mg·L^-1DNA template,0.125μmol·L^-1primers,0.2mmol·L^-1dNTPs,3.0mmol·L^-1Mg²⁺, and1.0U TaqDNA polymerase.According the result of SSR marker, the11kinds of Lycoris could be clustered into3groups,the third cluster was L.albiflora, and SSR primer pair17was selected to identify parentalmaterials and hybrids. While the11kinds of Lycoris were clustered into4groups for SCoT marker,and the third cluster was L.sprengeri.4. In vitro propagation system of Lycoris Herb.Rapid propagation systems were established using double bulb-scale of Lycoris radiata andLycoris chinensis as experimental materials and screened the best medium at every stage. The bestmedium of culture establishment stage was MS+6-BA1mg·L^-1+NAA0.2mg·L^-1+sucrose30g·L^-1and bulblet eproduction stage was MS+6-BA0.5mg·L^-1+2,4-D1mg·L^-1+sucrose30g·L^-1; MS+6-BA1mg·L^-1+2,4-D0.5mg·L^-1+sucrose30g·L^-1was benefit for plantlet-promoting and1/2MS+NAA0.5mg·L^-1+sucrose30g·L^-1was benefit for root induction.The rapid propagation system use hybrid embryos as material. The most ideal callusinduction medium of embryo culture was MS+6-BA1.0mg·L^-1+2,4-D0.5mg·L^-1, while the mostideal clustered shoots induction medium was MS+6-BA2.0mg·L^-1+2,4-D1.0mg·L^-1.