The Technology Of Mixed Pretreatment Of Straw And Dung And The Experiments Of Hydrogen Production Bv Photosvnthetic Bacteria

In the process of Photosynthetic hydrogen production, the productionmaterial is always one of the main problems that limits the development of thetechnology, due to its cheap and easy to get,Wood fiberis widely considered to be thegreat potential of the hydrogen production, through a variety of microbialfermentation,It can be transformed into sugar which can be directly used byphotosynthetic bacteria, Finally through Photosynthetic hydrogen production, into aclean renewable energy-hydrogen, Therefore, the degradation of the lignocelluloseconversion process is one of the most important research aspects. In addition, alongwith the sustained and rapid development of animal husbandry, Animal husbandrybreeds dimensions expand increasingly,and it’s pollution problems have becomeincreasingly serious, it has caught people’s attention and become our country and eventhe global pollution. Among them, especially in the livestock and poultry manure asthe major source of pollution. Therefore, to study how to solve the pollution causedby animal and poultry dung is imminent.This paper is based on research supported by the National Natural ScienceFoundation (No.50676029).Firstly,it research on the speciality of the rumen of largeherbivores,in which contains cellulose-decomposing microorganisms. Then with thecooperation of the effects in the process of decomposing cellulose by cellulose-decomposing microorganisms,it find a preprocessing method which can preprocessesthe cellulose by the increasing cellulose-decomposing microorganisms.This methodmixes the cellulose and dung of large herbivores in appropriate conditions, and makesthe cellulose-decomposing microorganisms grow quickly,at last,the cellulose can bedecomposed as reducing sugar content.In the procedure, the response surface analysismethod is used to design the experiments and analysis the results of the experiments.The results showed that:1.Through the experiments,the dung of dairy cattle really contains the cellulose-decomposing microorganisms.By the single factor experiments,it obtains that pH value,temperature and processing time has significant influence to the growing of the cellulose-decomposing microorganisms. Based on this fact,the three factors and three levels response surface analysis is made.At last,the results show that the best factors conditions which has been revised are5days,pH5.9and49℃, and its regression equation lists as follow: OD value=1.82-0.043X1-0.063X2+0.12X3+0.013X1X2-0.99X1X3-0.031X2X3-0.51X12-0.4X2-0.38X3.And the largest OD value is1.8284,which means the highest content of reducing sugar in the substrate is1.2804mg/mL.2. Based on the fact,the next experiments continue.The experiments mix the cellulose and dung of large herbivores in appropriate conditions. By the single factor experiments,it obtains that pH value,temperature, processing time and grain size have significant influence to the growing of the cellulose-decomposing microorganisms.The results show the best grain size is0.097mm. Based on this fact,the three factors and three levels response surface analysis is made.At last,the results show that the best factors conditions which has been revised are6.3days,pH4.9and48℃, and its regression equation lists as follow: ODvalue=2.67-0.062A-0.12B+0.18C+0.029AB-0.14AC-0.048BC-0.72A2-0.57B2-0.57C2. And the largest OD value is2.6263,and practical OD value is2.6263,and there is just small distinguish between them,which proves the regression equation is very useful.3.In the last step,many factor conditions are chosen to continue the hydrogen production experiments. Finally,the results show that the factors conditions which are forecasted by the response surface analysis are the best conditions.