The Application Of Traditional Chinese Medicine In Disease-resistance Of Litopenaeus Vannamei

Litopenaeus vannamei was one of the most economical shrimps in China. Shrimps lackedan adaptive immunity，so enhancing the immunity of shrimps would improve their resistance todisease infection. Research indicated that a number of traditional Chinese medicine（TCM）couldenhance the immunity of invertebrate. This research was carried on the effects of TCM ongrowth, feed conversion rate and innate immunity of L. vannamei from fed diets with thedifferent level.And aimed to enrich the fundamental researches of traditional Chinese medicineused as promoting its green and healthy development, while make TCM become scientific andtittle-used to come true.Healthy L. vannamei with weight of（1.44±0.30）g and length of（4.52±0.30）cm weredivided into5groups with3replicates. Feeding each group respectively with diets containing0%(control group),0.5%,1%,2%,3%of TCM for50days. Length were tested in company withweight at the last day. The results showed that the final weight and weight gain rate increasedwith increasing TCM, after attaining their maximum values，they decreased with TCM; of the5groups2%group had the highest final weight and weight gain rate，all of the test groups weresignificantly different compared to control group excepted0.5%groups (P<0.05). Throughquadratic model regression analysis between weight gain rate and the TCM levels in the dietsone regression equation was expressed as Y=573.704+180.524X-37.788X2（R2=0.99）.Themaximum weight gain rate was observed when the TCM level in diets was2.39%。Shrimps blood samples were collected from5groups when0th,3th,5th,10th,15thdays, fordetecting phenol oxidase(PO), peroxidase(POD), oxide dismutase (SOD) activity. The resultsshowed that the activities of PO, POD, SOD increased with increasing TCM, after attaining theirmaximum values，they decreased with TCM. On10thdays, the activity of PO of1%,2%,3%groups were significantly higher than control group（P<0.01）and2%group was the highest(0.0093U/mL), on the5thdays, the activity of POD and SOD significantly increased by1%,2%,3%groups（P<0.05）。The shrimps were infected by white spot syndrome virus (WSSV) after growth test，1000healthy shrimps were randomLy divided into5groups with2replicates and100shrimps in eachreplicate. Feeding each group respectively with diets containing0%(control group),0.5%,1.0%, 1.5%of traditional Chinese medicine for10days.The result showed that all the groups whichwere added with TCM had lower mortality than the control groups(P<0.05), and the relativepercent survival of treated groups from29.69and to61.98, the2%groups was highest（61.98%）。The results of full test showed adding some TCM to essential feed could not onlypromotedthe growth of shrimps, but also could increase the non-specific immunity function andthe premunition resisting power to disease. While taking the costs of raw materials intoconsideration, it suggested that the2.0%is the better level when apply to production andpractice.