Studies On Extraction Technics Of Chlorogenic Acid And β-carotene From The Arctium Lappa L.

Optimum extract conditions, purification and properties for the extraction of chlorogenicacid and-carotene from the Arctium Lappa L. were studied through orthogonal rumgyratecomposite experiment design and Response Suiface Methodology.The results showed that the optimum conditions for the extraction of chlorogenic acidfrom the leaves of Arctium lappa L. were as follows, the particle size was40, the ratio ofmaterial to solvent was1:21, ethanol concentration71%and temperature81℃, under suchcondition the extraction rate could be up to3.50%at twice extraction each for2h.Chlorogenic acid extractives were purificatied by resin HPD450column chromatography,and collected at different ethanol concentration, each named A, B, C. The collected samplesshowed better inhibitory effect on bacteria than mold and Saccharomycete. A, B and standardchlorogenic acid solution showed the same bacteriostasis on Escherichia coli. Mucor spp.andsaccharomycete, their contents of chlorogenic acid steadily increase. The crude extractiveshowed batter bacteriostasis on Staphylococcus aureus, Bacillus Subtilis, Rhizopus stoloniferthan Escherichia coli., Shiga bacillus, Mucor spp.and Saccharomycete. C showed the bestbacteriostasis than A, B and standard chlorogenic acid, especially on the Mucor spp.. Thestandard chlorogenic acid showed no bacteriostasis on the mold and saccharomycete. Refinedchlorogenic acid samples present instability under the conditions of different pH and oxidants.The optimum conditions for the extraction of-carotene from the roots of Arctium lappaL. were as follows, the extraction technique was ultrasonic aid organic solvent，the solventwas AC PE (12), the particle size of burdock was60, the ratio of material to solvent was123,42min, temperature49℃, extracted twice each for2h, under such condition the extraction ratecould be up to507.3μg/100g.