Studies On The Extraction And Character Of Inulin And Dietary Fiber From Arctium Lappa Linn

In this paper this four methods of hot water, microwave, ultrasonic wave and ultra high pressure to extract inulin from the Arctium lappa L., condition to extract DF from the Arctium lappa L. pomace were studied, And functions of DF from the Arctium lappa L. pomace were evaluated. The main results were shown as follows:Hot water to extract inulin from the Arctium lappa L.: Based on the single factor experiment, the orthogonal experimental design was employed in the work, the results showed that influencing factors of hot water extraction arranged in order were as follows: ratio of solid to liquid> time > temperature. The extraction rate was 31.29% under the optimum extracting conditions: ratio of solid to liquid(m∶V) 1∶2 5, time 105 min, temperature 60℃. Microwave to extract inulin from the Arctium lappa L.: Based on the single factor experiment, the orthogonal experimental design was employed in the work, the results showed that influencing factors of microwave extraction arranged in order were as follows: ratio of solid to liquid> microwave power> time. The extraction rate was 31.22% under the optimum extracting conditions: ratio of solid to liquid(m∶V) 1∶8, power 700 W, time 4 min. Ultrasonic wave to extract inulin from the Arctium lappa L.: Based on the single factor experiment, the orthogonal experimental design was employed in the work, the results showed that influencing factors of ultrasonic wave extraction arranged in order were as follows: ratio of solid to liquid> time> ultrasonic power> temperature. The extraction rate was 32.30% under the optimum extracting conditions: ratio of solid to liquid(m∶V) 1∶30, time 15min, temperature 60℃, ultrasonic power 420 W. Ultra high pressure to extract inulin from the Arctium lappa L.: Based on the single factor experiment, The extraction rate was 30.41% under the optimum extracting conditions: pressure300MPa, ratio of solid to liquid(m∶V) 1∶30, time 2min.The inulin extraction rates under the best of conditions of these four methods( hot water extraction, microwave extraction, ultrasonic wave extraction and ultra high pressure extraction) was compared, the results showed that: ultrasonic wave extraction (32.30%) > hot water extraction (31.29%) > microwave extraction (31.22%) > ultra high pressure extraction (30.41%). Using hydrochloric acid to remove impurities of Arctium lappa L. pomace The Purifing effects of on Arctium lappa L. pomace were studied.The optimal condition for purifying were hydrochloric acid concentration 5%, time 2 h, temperature 80℃, ratio of solid to liquid(m∶V) 1∶1 0. Treated in the conditions, the impurity removal rate of Arctium lappa L. pomace is 39.13%. After Arctium lappa L. purifying the insoluble dietary fiber amounted to 67.65%.The decoloring effect of H2O2 concentration, time, ratio of solid to liquid on dietary fiber were investigated using hydrogen peroxide as decoloring reagent. The optimal condition for decoloring were H2O2 9%, time120 min, ratio of solid to liquid(m∶V) 1∶20. Treated in the conditions, the hunter white of Arctium lappa L. dietary fiber is 67.81.