The Research Of A New Species In Grateloupia And A New Record Species In Gelidopsis On The Basis Of Morphological Observation And Molecular Analysis

When we carried out the analysis on a red algae which were collected in Xincun town,Lingshui country, Hainan province on the basis of morphology observation, rbcL sequenceanalysis and life history study, we found that:(1) thallus are purple, linear and cartilaginous intexture;(2) thallus with disc-shaped hapteron has a small short handle at base and constrictedat base;(3) the main branch are cylindrical or close to cylindrical; the small branch with two orthree pinnate branched are alternata or growing in the one side;(4) the tetrasporangiacruciately divided;(5) both carpogonial branch and auxiliary cell ampullae areGrateloupia-type;(6) there is no intergeneric divergence among of the four specimenscollected from Xincun town, Lingshui country;(7) they form a separate clade and appeared inthe midst of a large Grateloupia monophyletic clade in the NJ phylogenetic tree and MPphylogenetic tree;(8) they has a typical triphasic life history with isomorphic gametophyte andterasporophyte. So, we concluded that the red algae of our research is a new species whichbelonged to the genus Grateloupia and are named as Grateloupia lingshuiensis Wang et Luansp.nov. on the basis of morphological observations, rbcL analysis and life history.On the basis of morphology observation and rbcL sequence analysis, the red algae whichis collected in Chiling town, Lingshui country, Hainan province were researched. The resultsas fllows:(1) thallus are dark purple, linear, cluster and leathery in texture;(2) the mainbranches with irregularly fork branchlets are close to cylindrical or compressed; the endportion of the branches were wider and slightly flat;(3) there are adsorbers in the bottom of thealga which are used to adsorb cornerstone or entangle with each other;(4) the herbariums ofthe specimens do not attach to the paper;(5) the blades consists of cortex and medulla; themedulla consists of cylindrical cells with thick wall which are long, thin and small;(6) there isno intergeneric divergence among of the four specimens; there is1bp (0.3%) intergenericdivergence between the four specimens and Gelidopsis scoparia collected from Hawaii;(7) thefour specimens and Gelidopsis scoparia collected from Hawaii form a separate clade andappeared in the midst of a large Rhodymeniales monophyletic clade in the NJ phylogenetic treeand MP phylogenetic tree; the clade is the most close to Halichrysis coalescens, but thebootstrap is lower. Therefore, we concluded that the red algae of our research is the same species of Gelidopsis scoparia which belonged to Rhodymeniales and is preliminary identifiedas a new record species in China.Our research is contributed to the the system taxonomy and species diversity in theGrateloupia and Gelidopsis. At the sametime, through our research we provides someimportant reference information for the early development research, artificial breeding andgermplasm preservation of Grateloupia.