| Tilia amurensis Ruprecht is a secondary national protection plants with great economicbenefit and ecological benefit, but it is in danger now，the quantity and quality of Tiliaamurensis is on the decline; Juglans cathayensis Dode has a important medical value andeconomic value, studies is only limited in the filed of medicine now. In this paper, geneticdiversity、genetic structure、genetic relationship was analysed by the means of AFLPflorescence labeling in order to provide the reference to valuation、collection、protection ofTilia amurensis and Juglans cathayensis. The result as follows：1. Genetic diversity of Tilia amurensis:9primer combinations selected from64primercombinations produced a total of1374fragments，1370of which were polymorphic with apolymorphism percentage of99.70%. The range of Na、Ne、H、I respectively were1.9922-2.000,1.2738-1.3957,0.1802-0.2446,0.2986-0.3836. This result indicated that Tiliaamurensis had a high genetic diversity. Genetic similarity coefficient（SC）of the62singleplants changed from0.3911to0.7953with the average was0.5761. All the single plants wereclustered into12groups with UPGMA at0.60, and the plants originated the same place didn’tdistribute in the same group totally. It indicated that there was no strict relevance between thegenetic diversity and geographic origin of Tilia amurensis.182specific bands including6absent bands were amplified from51single plants，which accounted for13.3%of totalpolymorphic bands.82.3%of the Tilia amurensis single plants could distinguished by thespecific bands. Polymorphism percentage of5Tilia amurensis populations changed from38.68%to81.58%with the average was60.33%and99.69%at the species level. The order ofpolymorphism percentage from big to small was: Mountain Tai Hou Shiwu＞Kun YushanHuanshan Road>Penglai Nan Guanshan>Lu shan Yulin>Lao shan Bei Jiushui.2. The genetic structure of Tilia amurensis: Gst was0.1545, it suggested geneticvariation was mainly within the populations, Nm was2.8324; Genetic identity ranged from0.9460to0.9935with the average was0.9654; Genetic distance changed from0.0183to0.0557with the average was0.0366, the genetic similarity coefficient was high ininterpopulation; All the populations were clustered into3groups at0.035, and that there wasno strict relevance between the genetic distance and geographic origin of the Tilia amurensis.3. Genetic diversity of Juglans cathayensis:1141bands wrere amplified from total genomic DNA of Juglans cathayensis with eight primers，in which there were1124polymorphic bands, the number of polymorphic bands ranged from124to160due todifferent primer combinations with a polymorphism percentage of98.5%. Na、Ne、H、I inaverage respectively were1.9845、1.3384、0.2095、0.3336.179specific bands (including10absent bands) were amplified from44single plants.75.9%of the Juglans cathayensis singleplants could distinguished by them. Polymorphism percentage of5Juglans cathayensispopulations changed from51.92%to80.12%with the average was58.57%and98.45%at thespecies level. The order of polymorphism percentage from big to small was a little differentamong PPB、H and I, and the final order was: Mountain Tai Fo Yesi＞Lao shan Bei Jiushui＞Lu shan edge of the core zone>Kun Yushan Nan Tianmen> Mountain Tai Bo Geya.4. The genetic structure of Juglans cathayensis: Gst was0.1722, which indicated thegenetic variation in interpopulations accounted for17.22%of total genetic variation, and Nmwas2.4738, which showed there was a contaction among different populations. Geneticidentity raged from0.9494to0.9721with the average was0.9573. Genetic distance rangedfrom0.0283to0.0522with the average was0.0437. Based on the UPGMA cluster diagram,the5populations may be divided with genetic distance of0.043into3groups. The resultdidn’t showed there was a relevance between genetic distance and geographic origin ofJuglans cathayensis. |
PDF Full Text Request