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Study On Cold Resistance Of Miscanthus Lutarioriparius

Posted on:2014-06-10Degree:MasterType:Thesis
Country:ChinaCandidate:X Y DuFull Text:PDF
GTID:2253330425491079Subject:Genetics
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In this study, physiological characterics and regrowth experiment of five M. lutarioriparius were researched under the low temperatures. In addition, conductivity content, MDA, SOD, POD, praline content, Soluble sugar content, soluble protein content were measured through low temperature gradient so as to study their abilities. And the main conclusions were as follows:1. The semi-lethal temperature of these were analysed through the measurement of the relative conductivity and the calculation combining with the Logistic equation and the results of B0121、A0110、B0120、A0107、B0145were-6.97、-6.51、-6.34、-5.00、-3.26in correspondence.2. The cold resistance of them was primarily studied by the means of electrical conductivity method and regrowth experiment. The rhizome of all samples could not survive the temperature at-10℃. All samples could recover after exposure to0℃, these results were as same as the semilethal temperature LT50.3. The variation trend of a same physiological index of different M. lutarioriparius species was almost unanimously with low temperature stress. Soluble sugar content, soluble protein content, conductivity content, MDA, SOD, POD, proline content increased with stress temperature dropping, but activity first increased and then decreased and its corresponding temperature of different species was different.4.The result of ANOVA showed that the electric conductivity, the soluble sugar content, the soluble protein content, POD, SOD, proline content and the MDA total content were significant different under the different low-temperature treatment and the different species.5.The cold-resistance ability of five M. lutarioriparius, from higher to lower, was B0121>A0110>B0120> A0107>B0145, by subordinate function values analysis.
Keywords/Search Tags:Miscanthus lutarioriparius, low temperature stress, physiologicaland biochemical indicators
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