| Miscanthus lutarioriparius and Miscanthus sinensis are two different species of Miscanthus spp.,which are widely distributed in China and have good energy-related quality traits.M.lutarioriparius is a Chinese native species and distributes in the area of 4°~28°N,111°~119°E.It mainly distributes in the middle and lower reaches of Yangtze River,including the provinces of Hunan,Hubei,Jiangxi,Anhui etc,.M.lutarioriparius has a series of excellent traits which the energy crops should have,including high biomass yield,high cellulose,hemicelluloses and lignin content.However,it usually requires high input of water and fertilizer.M.sinensis mainly distributed in the area of 18°~43°N,97°~126°E in China,including the provinces of Hunan,Hubei,Jiangsu,Guizhou,Hainan,Jiangsu,Jilin,Shandong,Liaoning etc.It has the characteristics of high yield,strong ability of soil restoration,strong alkali resistance and low water and fertilizer requirement.As the promising cellulose energy plant,these two species have some complementary traits.Therefore,it is expected to create some excellent hybrids with good traits by crossing these two species.This study firstly created 215 hybrids which were crossed and reciprocal crossed between M.lutarioriparius and M.sinensis.Using these materials,the following work was conducted:1)the genetic model of the traits was created using the poly gene mixed genetic analysis,including the traits of tiller number,axillarybud number,dry weight,maximum culm length,base outer diameter,base inner diameter,middle outer diameter,the middle inner diameter;2)hybrids which can be used for different utilization method were screened using the grey related analysis.Results showed that:(1)The variation of the eight measured traits of the hybrids was higher than that of their parents,especially the traits of diameter of basic stem,axillarybud number and dry weight of plant.(2)Significant differences were observed between two reciprocal crosses in the traits of tiller number,axillary bud number,plant height,inner diameter of basic stem,outer diameter of middle stem and inner diameter of middle stem,which suggested that there should be maternal genetic phenomenon for these traits in Miscanthus spp..(3)The genetic model of the axillary bud number was fitted to the two additive-dominance major gene model(B-2),and the heritability of the major genes of crosses and reciprocal crosses were 86.32%and 92.37%,respectively.The A-0 genetic model was the most suitable model for the traits of dry weight of plant,plant height outer diameter of middle stem.The tiller number was controlled by one major gene model.The inner diameter of basic stem and inner diameter of middle stem were controlled by two major gene model.The outer diameter of basic stem was suitable for A-0 model and one major gene model in crosses and reciprocal crosses,respectively.The results of this paper afford a theoretical basis for the further study of QTL mapping of agronomic traits and molecular marker assisted breeding program in Miscathus spp..(4)The material of hybrid 4~#,hybrid 69~#,hybrid 3~#and hybrid 29~#were screened as excellent materials for biogas generation,with a grey relational analysis score of 0.762,0.718,0.702,0.698,respectiely.(5)The material of hybrid 4~#,hybrid 3~#,hybrid 18~#and hybrid 69~#were screened as excellent materials for direct combustion,with a grey relational analysis score of 0.745,0.723,0.720,0.692,respectiely.(6)The suitable ethanol production strain was screened and the hybrid 4~#(0.716)was the highest,followed by hybrid 3~#(0.695),hybrid 69~#(0.685)and hybrid 120~#(0.666).Combining the above results,the hybrid 4~#was the best material for both the utilization of biogas fermentation,direct combustion and ethanol fermentation. |
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