| Lycium barbarum L. is a perennial shrub, its fruits, known as medlar, is used as a common medicinal material in china. Because of their luxurious growth of stems and leaves, nutrient and sweet of fruit juice, they had emerged so many faults such as diseases and insect pests. The vegetative and reproductive growth of wolfberry carried on simultaneously, and it’s blooming and fruiting at a same period. As the period of young fruit is long while the mature enlargement period is short, it’s extremely difficult to prevent insect pests. Pesticide prevention can hardly achieve the expected effect, or easily lead to serious pesticide residues because of the pesticide abuse. Nowadays, the studies of wolfberry’s pest management are concentrated on the bioassay and efficacy trial of chemical pesticides, but the application research of Bionic glue as a novel physical control on the wolfbeny’s pest management, and its safety evaluation have not been reported.In this research, the influence of Bionic glue on the LBP content of medlar, the growth and chlorophyll fluorescence of wolfbeny’s leaves, and the biodegradability of Bionic glue were investigated to estimate the safety of Bionic glue.To estimate the control efficiency of Bionic glue on the pests and to set a good example for other integrated pest management (IPM). Effects of Bionic glue on the behavior of adult Paratrioza sinica Yang&Li and the adhesion of Bionic glue on P. sinica, Psilothrips indicus, Lema decempunctata Gebler were preliminary studied. In addition, the control efficiency on the main pests and some overwintering pests were also investigated in field condition. The main results are as follows:(1) The extended leaves of wolfberry which had been sprayed by Bionic glue at their frondescence at early spring were sampled to observe the changes of leaf epidermis by paraffin slice and microscopic observation. Results:there was no significant change displayed on the cell structure for the cross-section and epidermal cells of wolfberry leaves, meant that the application of Bionic glue would not obviously effect on the growth of wolfberry leaves.(2) The photosynthetic performance of wolfberry leaves under Bionic glue treatment was investigated by IMAGING-PAM. Results:each chlorophyll fluorescence parameter showed its own characteristics, but no significant difference between each parameter after Bionic glue treatment.(3) Being spayed by Bionic glue at early spring, the wolfberry fruits were collected at summer and autumn for measurement of LBP and microelements. The microelements in the soil collected at summer were detected as well. Results:The LBP of fruits and the microelements content in soil and fruits were not significantly changed after the treatment, and all the results were consistent with the requirement of Chinese Pharmacopoeia (2010).(4) The behaviors of adult P. Sinica and differential prevention effect on adult P. sinica, L. decempuncata and P. indicus response to Bionic glue treatment were studied. Results:The Bionic glue had strongly adhesion to adult P. Sinica. For the chewing insect pests like L. decempuncata, the Bionic glue sprayed on wolfberry leaves blocked or sticked their mouthparts when they chewing the leaves, and which caused their death. For the P. indicus, the controlling effect was not notable, which might due to the behavior and tarsus structure of P. indicus and needed a further study.(5) The population of the major insect pests on wolfberry and their natural enemies were investigated in a period of time after Bionic glue and pesticide treatment. Results:the effect of Bionic glue on controlling effect were different according to different species:the population of P. sinica, Jaapiella sp. and Aphis sp. were effectively reduced at early stage of Bionic glue application, but later as there was no partition between the treatment group and control and the new leaves grew, the Bionic glue invalidated as it depredated. For P. indicus, the control effect of Bionic glue was equal to pesticide. While for A. pallida, the controlling effect was significantly, throughout the entire growing season.(6) The overwintering behavior of P. sinica and A. pallida were investigated by the method of sprayed stems with Bionic glue. Results:Bionic glue could be used to block the P. sinica and A. pallida when they seek for overwintering sites.(7) Bionic glue was found easily biodegradable by the microbe in experimental conditions by the observation of medium and the production of carbon dioxide (PCD). |
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