| Anthurium andraeaum is a perennial evergreen herb which belongs to Anthuriumgenus of araceae with beautiful mosaic, elegant unique flowers. It has higher economicreturns and enormous market size. Due to traditional breeding methods can’t meet themarket demand, plant tissue culture technology has been widely used in the massproduction of seedlings. The regeneration system has been established basically. But theestablished regeneration system is low efficiency with poor seedling quality. In order toestablish a high quality and efficiency propagation technology of Anthuriumandraeanum plantlets, in this study, experimental materials was four new varieties ofpotted Anthurium anthurium. Axillary tissue culture, proliferation and strong seedlings,rooting conditions and somaclonal variation in tissue culture process weresystematically analysis, the main results were as follows:1. Axillary initial culture results showed that, using a single method of sterilizationis difficult to completely sterilized. Used0.1%HgCl2in10min, inoculated in normalmedium contamination rate was about91.84%, as well as inoculated in acidifiedmedium between35.26%to45.38%. Medium supplemented with L-A100mg·L-1-400mg·L-1can control contamination rate in22.00%-24.80%. With the increase of theL-B concentration, the contamination rate gradually declined from45.00%to17.74%.Secondly,6-BA is more suitable than ZT in the axillary bud differentiation, IBA isbetter than NAA. The axillary bud can differentiation directly in the medium1/2MS+6-BA1.0mg·L-1+IBA0.1mg·L-1.Thirdly, the sprout axillary bud was better than othertwo kinds in differentiation ability.2. In the process of subculture, the light source has an important impact on thegrowth of SYN01-L. The results showed that plantlets cultured under traditionalfluorescent light not as robust as LED lights. For SYN01-L is LED lamps V1.Theaverage number of leaves per plant reached4.7with height2.8cm, the callus was up to1.16cm, the number of adventitious buds was1.53, fresh weight reached the maximumwas1.0439g with the highest chlorophyll content. 3. Using the SYN01-L’s single bud as subculture explants, results showed thatauxin IBA was superior than NAA,2,4-D in proliferation. ZT2.0mg·L-1or6-BA2.0mg·L-1combine with auxin IBA was the most ideal medium for differentiation andproliferation, the average differentiation reached4.69and4.43. Secondly cytokinin canbe decreased when strong seedlings. Seedling index increased significantly from0.484to1.189with cytokinin ZT reduced to0.5mg·L-1and added IBA0.1mg·L-1. Thirdly,strong seedlings could be choosed after cultivation during subculture.4. The single factor experiment showed that IBA had better effect than NAA.NAA、IBA、active carbon and sucrose were choosed in the orthogonal experiment whichoptimized the rooting way for the plantlet. The results of orthogonal experiment showedthe medium added with NAA0.2mg·L-1,IBA0.5mg·L-1,active carbon0.5g·L-1,sucrose30g·L-1was the optimal combination.5. The survey of somaclonal variation showed the mutation rate of the plantletclones was0.042%on the eighth generation and increased to0.076%on the tenth.Besides, with the increase of cytokinin action in initial culture, somaclonal variationwas also increased. Different varieties of variant strains had difference in morphology,and some traits can be inherited stably. Lastly cytokinin especially high concentrationwould increase the abnormality rate and the number of abnormal buds differentiationincreased sharply. |
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