The Study On Electrical Stimulation On Skeletal Muscle Neuropeptide Y (NPY) And Pelvic Floor Muscle Strength Recovery

Objective1. To evaluate the expression of neuropeptide Y in plasm and pelvic floor musclesamong diabetic rats and postpartum stress urinary incontinence rats before andafter treatment of electrical stimulation, through the observation of the changes ofpelvic floor muscles tention and neuropeptide Y among the diabetic rats andpostpartum stress urinary incontinence rats to explore the action and meaning ofNPY in pelvic floor dysfunction.2. To compare the neuromuscular morphology and pathological features among thepostpartum stress urinary incontinence rats and diabetic rats after electricalstimulation to povide the basis for the physical therapy in the treatment of pelvicfloor dysfunction.Methods1. To develope the type2diabetes rat model by high-fat high-sugar diet combinedlow dose STZ and the stress urinary incontinence rat model by imatating birthtrauma, and explore its characteristics.2. To compare the tention of pubococcygeal muscle.neuromuscular morphologyand the expression of neuropeptide Y in plasm and pelvic floor muscles beforeand after electrical stimulation. Results1. Before the electrical stimulation, the NPY concentration in Plasm among urinaryincontinence group and diabetes group were significantly lower than the controlgroup (P<0.05).2. After electrical stimulation, the NPY concentration in plasm among urinaryincontinence group and diabetes group were signicantly higher than before(P<0.05).however, there is no significantly difference in control group.3. Before the electrical stimulation, the contraction force among urinaryincontinence group and diabetes group were significantly weaker than the controlgroup (P<0.05).4. After electrical stimulation, the contraction force among urinary incontinencegroup and diabetes group were signicantly stronger than before (P<0.05),however, there is no significantly difference in control group.5. In urinary incontinence group and diabetes group, the expression of NPY inplasma was correlated with the muscle strength before and after the electricalstimulation (P<0.05).6. In urinary incontinence group and diabetes group, the expression of NPY inpubococcygeal muscle was correlated with the muscle strength before and afterthe electrical stimulation (P<0.05).Conclusions1. In the research of the type2diabetes rats and postpartum stress urinaryincontinence rats, the NPY concentration in plasma decreased, NPY can be usedas an indicator of a response to nerve damage.2. After the electrical stimulation treatment, the increased concentration of NPY indiabetes rats group and postpartum urinary incontinence rats group were relatedto the recovery of the pubococcygeal muscle strength, which maybe related toNPY in promoting the generation of the pelvic floor muscles, blood vessels.3. After the electrical stimulation treatment, the increased expression of NPY in pubococcygeal muscle among diabetes rats group and postpartum urinaryincontinence rats group were related to the recovery of the pubococcygeal musclestrength, we considered electrical stimulation increased the release of NPY as aneurotransmitter which reflects the recovery of the damaged nerve to increase themuscle strength.4. The pubococcygeal muscle morphology among type2diabetes rat model andpostpartum stress urinary incontinence model have appeared myogenic changesand the performance of neurogenic myopathy, which maybe good simulated theetiology of the pelvic floor dysfunction.