Effect Of Resveratrol On Autophagy In Prostate Cancer PC-3Cells

Objective: To observe autophagy of prostate cancer PC3cells induced by resvera-trol and explore the possible mechanism．Methods: l. Prostate cancer cell line PC-3was incubated in culture medium in vitro，using cell counting kit-8assay to detect the growth rate among different resveratrolconcentration groups (0、20、40、80、160μmol/L) and different time groups(24、48、72h)．2. Divided into control and experimental group，extracting total RNA of eachgroup，assessing the integrality and content of RNA，the level of LC3、Beclin1mRNA expression was examined by semiquantitative RT-PCR technique in the PC-3cells treated before and after with resveratrol．3. The expression of LC3-Ⅱ protein inPC-3cells treated by resveratrol was detected by Western blot.Results:1. CCK-8assay results：Within the concentration of (20-160) μmol/L，resveratrol can obviously inhibit proliferation of PC-3in vitro．The inhibition ratiowas stepping up as the concentration increasing，and the highest inhibition ratio wasin the group with the concentration of l60μmol/L and72h．There was statisticalsignificance of inhibition ratio in different concentration groups of PC-3cells afterbeing treated resveratrol (P<0.05)．2. RT-PCR detection results：There was differentelevation of the LC3、Beclin1mRNA expression in different concentration groups ofPC-3cells after being treated resveratrol．Resveratrol obviously elevated the LC3、Beclin1mRNA expression in PC-3at l60μmol/L， and there was statisticalsignificance between different groups (P <0.05)．3. The result of the Western blotshowed that after treatment with different concentration of resveratrol for24h, thelevels of LC3-Ⅱproteins expression were up, and there was statistical significancebetween different groups.Conclusion:1. We comfirmed that resveratrol had the capability of inhibiting theproliferation of PC-3cells in vitro by a dose and time-dependent manner．2. Within acertain drug concentration, resveratrol can inhibit the mRNA and protein expression of LC3and beclin1in PC-3cells．3. Resveratrol may induce autophagy leading cellsdeath to inhibit proliferation of prostate cancer cells.