Relations Of HPV Infection With Breast Cancer And C-erbB-2Expression In Breast Cancer Tissues

ObjectiveInvestigate the relations of human papillomavirus (HPV) infection with theclinicopathologic characteristics of invasive ductal carcinoma (IDC) patients and itscorrelation with the proto-oncogene c-erbB-2expression in the tumor tissues of thepatients.MethodsRandomly selected from January2008to January2012, the Fuzhou GeneralHospital general surgical resection of60cases of female invasive ductal carcinoma,and collected some of the clinical and pathological features of these patients,including histological grade, axillary lymph node metastasis, the maximum diameterof the tumor, the age and menopause. At the same time, we randomly selected fromthe same period in the surgical removal of30cases of breast fibroadenoma tumor andadjacent normal breast tissue (away the tumor≥3cm) as a control. HPV DNAdetection and typing were performed by surface Plasmon resonance (SPR) in thespecimens of IDC breast tissues (60cases), fibroadenoma breast tissues (30cases)and normal breast tissues (30cases). Meanwhile, the C-erbB-2protein expression inthe IDC breast tissues was detected by immunohistochemical staining.Results1. Four kinds of type HPV including HPV16, HPV18, HPV33, HPV59weredetected in invasive ductal carcinoma of the breast tissue, breast fibroid tissue andtumor adjacent tissues. All of them are high-risk HPV and low-risk HPV or mixedinfection was not detected.2. The HPV infection rate in IDC breast tissues was36.7%(22/60), in which theHPV33accounted for59.1%of the total HPV positive cases (13/22). The HPVinfection rate in fibroadenoma breast tissues and normal breast tissues group was 13.30%and3.33%respectively, which was significantly lower than that in IDC breasttissues (X2=5.3000, P=0.021).3. HPV infection in the IDC tissues was associated with the histological gradeand axillaries lymph node metastasis of the patients (both P<0.05). However, therelated differences of the largest tumor diameter, age and menopause are notstatistically significant (all P>0.05).4. There was a positive correlation between HPV infection and C-erbB-2proteinexpressions in IDC breast tissues (r=0.486; p<0.001).Conclusion1. HPV infection contributes significantly to the pathogenesis of IDC, and alsoclosely related to the degree of differentiation and invasive ability of the tumor.2. HPV infection participates the occurrence and development of breast cancermay probably by promoting the high expression of C-erbB-2.