| Inhalation Anesthetic preconditioning can produce early phase the role of anti-ischemic reperfusion injury (early protection the early phaseof protection EP), the delayed phase of the anti-ischemic reperfusion injury is unclear (delay protection delayedphase of protection, DP). Compared with the EP, DP not only can reduce myocardial infarct size in ischemia and reperfusion, but also can produce the anti-myocardial stunning and antiarrhythmic effect of DP pretreatment also repeated stimulation appear that prolonged ischemia and reperfusion24h given pretreatment generate resistance to injury, and2to3days and then give the pretreatment, its resistance to injury could occur. DP than EP broader clinical application prospects. Suppose given immediately before the donor heart preservation heart pretreatment, and its resistance to injury only embodied in the surgery did not reflect saved. Pretreatment given24hours before the donor heart preservation, can reflect myocardial resistance to injury in cardiac surgery and during the donor heart preservation. Inhalation anesthetic drug delayed phase of myocardial protection have important clinical significance.Objective:Explore the protective effect of isoflurane delayed preconditioning phase on the effect of cold preservation of isolated rat hearts.Methods:SD rats (n=36), depending on whether intraperitoneal injection of isoflurane were randomly divided into experimental group and the control group, thoracotomy in isolated rat heart, moved to the the Langendorff perfusion device on KH solution aortic perfusion, the heart beat reaches steady state, Hemodynamic basis of value and specimens from coronary effluent samples. KH solution equilibrium30min continuous perfusion after aortic root single intravesical4℃HTK solution, the surface of the heart at the same time cooling after cardiac arrest in the HTK solution saved, saved during re-perfusion preservation solution. Cold preservation time, divided into4h,6h,8h group, n=6. Save after re-moved rat heart perfusion KH solution on the Langendorff apparatus, gradually complex temperature to37℃, the cardiac resuscitation infusion30min, determination of hemodynamic parameters and specimens from coronary effluent measurement of cardiac enzymes. Compare saved recovery of cardiac function before and after coronary effluent creatine kinase (CK), lactate dehydrogenase (LDH) emission changes.Results:With the growth isolated heart to save time, indicators of cardiac function recovery rate is gradually reduced.4h,6h experimental group and the control group, the cardiac function of recovery rate between the significant difference (P>0.05), in which the the8h experimental group and the control group, the various indicators of cardiac function recovery rate difference was significant (P<0.05). Every group balance vitro heart preservation perfusion the coronary effluent foundation value, and no significant differences among the groups. With storage time isolated heart, each set of cardiac enzymes CK of LDH have increased to varying degrees, both the experimental group and the control group, group, compared with a significant difference (P<0.05), which between the experimental and the control group cold preservation4h and6h There were no significant differences between the experimental and the control group cold preservation8h significant differences (P<0.05). Prompted isoflurane preconditioning can improve in vitro heart preservation effect, but no significant differences in the cold preservation within6h.Conclusions:Cold preservation8h myocardial energy storage is a sharp decline in heart function significantly diminish the critical stage, the effect of myocardial protection of HTK solution within6h, while the experimental index slightly decreased, but the difference was not statistically significant, more than a child saved effect decline obvious. Isoflurane delayed preconditioning on cold preservation8h has a protective effect in isolated rat hearts, can significantly improve the the after saving heart function, reduce myocardial cell damage. Cardiac cold preservation technology. |
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