Simvastatin Serum Inflammatory Factors And Vascular Endothelial Effects Of Low Blood Sugar In Diabetic Rats

Background:Diabetes is a common and frequently-occurring disease, as people’s living standards improve, lifestyle changes, the increasing aging population, the incidence of diabetes is also increasing year by year, it has become after cardiovascular and cancer the third largest non-communicable diseases. Numerous studies confirm that type2diabetes mellitus (DM2) is an autoimmune disease, and low-grade inflammation in their occurrence and development, inflammatory factors and fat endocrine, oxidative stress, the immune system interact with insulin resistance and cell structure dysfunction and lead to DM2. Hypoglycemia is a common intensive treatment of diabetes pathological state, hypoglycemia its adverse effects on the cardiovascular performance is caused by myocardial ischemia and arrhythmias occur in patients with diabetes, low blood sugar may exacerbate the damage to the cardiovascular, low blood glucose and blood sugar fluctuations closely related great harm, but its cause damage to the body of the specific mechanism has not been elucidated.Object:By observing the impact of simvastatin on serum levels of inflammatory factors in diabetic rat hypoglycemia and the changes of the total antioxidant capacity, we can explore the diabetic rats hypoglycemia when changes in peripheral serum levels of inflammatory mediators and the. protective effect of simvastatin to the vascular endothelium of diabetic rats hypoglycemia.Method:There are54of male Wistar rats, randomly divided what into normal feeding group (NC)18and diabetic group (DM)36.The NC group is normal feeding while DM group establishing diabetic rat model. After the success of the model, the DM group were randomly divided into two sets of DA,and DB the DA group row is gavage with simvastatin. After4weeks,it will induce all the rats acute hypoglycemia. Three groups of rats are according<3.0mmol/L of blood glucoseor as hypoglycemia standard. Animals in each group were tail vein blood in the fasting state,3000r/min centrifuge immediately centrifuged for10minutes, separating of the serum, in order to detect indicators.Rats in each group were randomly divided into two groups,60mg/kg,120mg/kg intraperitoneal injection of insulin, blood glucose>2mmol/L as mild hypoglycemia,<2mmol/L for moderate hypoglycemia, and then to3%pentobarbital intraperitoneal anesthesia,2hours after the induction of hypoglycemia as abdominal aortic blood with3000r/min centrifuge serum hs-CRP and IL-6levels detected immediately before and after the induction of hypoglycemia and total antioxidant capacity. Highly sensitive enzyme-linked immunosorbent assay (ELISA) measurement of hs-CRP, IL-6Determination Application enzyme immunoassay (ELISA) method. The statistical analysis of the data:All data are expressed as mean±standard deviation, and analyzed using analysis of variance, the two groups were compared using the t-test, data processing and statistical analysis using SPSS13.0statistical package. P<0.05was considered significant.Results:Before hypoglycemia the NC group with the DA group have no significant difference (P>0.05)In the indicators IL-6and hs-CPR and total antioxidant capacity. NC group comparing with the in the DB group,IL-6and hs-CPR and total antioxidant capacity were significantly different (P<0.05),DA group comparing with the DB group, IL-6and hs-CPR and total antioxidant capacity are also significant differences (P<0.05).Mild hypoglycemia NC group、DA group DB group IL-6, hs-CPR were higher, the NC group, DA group increased total antioxidant capacity, total antioxidant capacity of the DB group decreased.IL-NC group with the DA group6. hs-CPR has no significant difference in total antioxidant capacity statistically different, the DA group DB group IL-6, hs-CPR, total antioxidant capacity were different;Moderate hypoglycemia DA group and NC group, IL-6, hs-CPR and total antioxidant capacity are significant differences,the DA group comparing the DB group IL-6and hs-CPR and total antioxidant capacity were significantly different(P <0.05).Conclusion:1As blood glucose levels lowing in diabetic rats serum inflammatory factor levels increased gradually the total antioxidant capacity reduced;2. Simvastat in gavage group rats has low glucose serum levels of inflammatory mediators and high total antioxidant capacity than that gavage group.3Simvastatin in diabetic rats with acute low blood sugar, anti-inflammatory, antioxidant, protecting vascular endothelial role.