Chemical Basis Research On Hpyerglycemic Effective Part Of Radix Paeoniae Rubra

Radix paeoniae rubra is a traditional Chinese medicine for the treatment of blood stasis syndrome since ancient time. The main purpose of the present paper is to separate components from the effective part that we enriched, and to study the hyperglycemia effect of enriched part. At the same time, we hope to find new compounds, which could support clinical reuse, and provide certain theoretical basis of radix paeoniae rubra for the treatment of "promoting blood circulation to remove blood stasis".This study consists of three parts.(1) The extraction and enrichment of radix paeoniae rubra effective part. Based on previous experiment, the procedures of extraction were determined as following:First of all, extraction with95%ethanol once, and then by60%ethanol twice, solvent was recovered respectively under reduced pressure to obtain the extraction. The effective part was enriched by column chromatography with Sephadex LH-20as solid phase.(2) The hyperglycemia effect of the enriched part was evaluated on KK/Upj-Ay mice. These animals were separated in to six groups including high, medium and low groups of the enriched part which doses were800,400,200mg/kg·d-1,3mg/kg·d-1of rosiglitazone positive group, as well as vehicle and model groups, with vehicle and model groups giving the same dose of solvent. Lavage administration for3weeks, the result showed that the middle dose group of the enriched part could lower blood glucose, which had significant difference comparing with the model group (P<0.05), and it’s hypoglycemic effect is better than rosiglitazone, but showed no significant statistically difference (P>0.05). The result of insulin sensitive index detection showed that enriched part of low, medium dose group could increase the insulin sensitive index, but showed no significant statistically difference comparing with model group (P<0.05).(3)27compounds were isolated by complex utilizing column chromatography of silica gel, ODS, solvent extraction, recrystallization, and the preparative ODS silica gel column chromatography to isolate compounds from the enriched part. The chemical structuresof these compounds were identified using UV, ESI/MS, ID NMR and2D NMR analysis technology. As a result the structures of24compounds were elucidated successively. These compounds were6monoterpene and it’s glycosides:4-ethyl-benzoyl paeoniflorin(1), Epi-4-ethyl-benzoyl Paeoniflorin (2), Paeoniflorin (3), Hydroxy paeoniflorin (4), Benzoyl paeoniflorin (5), Albiflorin (6) and Paeoniflorigenone (7);3nucleosides:2-amino adenosine (8), Adenosine (9),1-aniline-2-carboxyl-5’-glycerol adenosine (10);10tannins:3,4’-O-dimethyl ellagic acid (11),3,3’-O-dimethyl ellagic acid (12),3,4,4’-O-trimethyl-5-hydroxy-5’-methyl ellagic acid (13),3,3’,4-O-trimethyl ellagic acid (14), Gallic acid (15), Methyl gallate (16), Ethyl gallate (17), Butyl gallate (18);3flavonoids:Dihydroquercetin (19), Dihydrokaempferol (20), Naringenin (21),1,2,3,4,6-O-pentagalloylglucose (22),1-O-galloylglucose (23);3other types of compounds:Benzoic acid (24), Glycerin (25), Ribose (26), Dibutyl phthalate (27). Compounds1,2,10,13were two new compounds, and have not been reported before.8-14,18-20,23,25-27were isolated from radix paeoniae rubra for the first time, nucleosides which represented by compounds7-9have not been reported before in the genus.