Research Of Ginkgo Biloba Extract Temperature-Sensitive Nose Situ GEL

Objective Study on extract of Ginkgo biloba leaf temperature-sensitive in situ gel for nasal determination,process of preparation and release in vitro,security and stability,and pharmacodynamics.Methods Establishment of HPLC-UV determination of content of total flavonoids in extract of Ginkgo biloba leaves.Chromatographic columns for Diamonsil C18, mobile phase for methanol-0.4%phosphate (55:45,V:V), flow rate:0.8mL·min-1detection wavelength:360nm;Column temperature:30℃, and the sample size:20μL. Establishment of HPLC-ELSD determination of terpene lactones in extract of Ginkgo biloba leaf content.Chromatographic columns for DiamonsilC18column;mobile phase:methanol-tetrahydrofuran-water (25:10:65, V:V:V);Flow rate:0.8mL·min-1gas flow rates:2.7mL·min-1;column temperature:35℃; injection volume:10μL;drift tube temperature:115℃.In extract of Ginkgo biloba leaves through the determination of flavonoid and terpene Lactone content determination method for establishment of Ginkgo biloba extract. Viscosity index,orthogonal experiment to determine the best gel preparation process. Study on Ginkgo biloba extract in situ gels release.Experimental study on Ginkgo biloba extract a dialysis membrane-less nasal in situ gel dissolution;to biofilm (bovine,ovine nasal mucosa) dialysis test and simulation analysis of the human nasal mucosa for drugs;in dialysis bags experimental study drug’s molecular weight (size) for effects enter the body,to study of Ginkgo biloba extract in situ gel releases study.Preparation of3batches of extract of Ginkgo biloba leaves by in-situ nasal gel,test,light test,high temperature and high humidity test to examine the stability of Ginkgo biloba extract in situ gel for nasal.Use scopolamine hydrobromide in mice induced by impact memory barriers,adverse effects on memory consolidation induced by sodium nitrite, effects on ethanol-induced impairment of memory in mice reproduce efficacy.Results Methodological study,determination method is simple,easy,fast and accurate.By orthogonal experiments,Determine poloxamer188:poloxamer40715:20,0.4%Chitosan,30%HP-P-CD is the best preparation technology.Through the in vitro release studies,indicating that the.drug is spread into the body rather than by gelatin dissolving drug release.Different doses of Ginkgo biloba extract in situ gel to improve learning and memory dysfunction. Conclusion Test results indicate that temperature-sensitive in situ gel of extracts of Ginkgo biloba leaves with good sustained-release effect,by the nose-channel directly into the brain of the brain play a role,you can make the drug is absorbed more efficiently,improve bioavailability, conducive to patient care in the case of equal efficacy of intranasal oral dosage can be reduced, lower drug costs.