The Temperature Sensitive Ginkgo Biloba Extract Nasal In Situ Gel Research Report

Ginkgo biloba is a gymnosperm Ginkgoaceae,is one of the world's oldest tree species,is listed as the two national protected plants,also known as a "living fossil".Research shows that,the leaf and fruit have important medicinal value."Compendium of Materia Medica" in the records of taste sweet and astringent,and have asthma,enuresis,reduce it,check poison insecticidal effect.The "Chinese Pharmacopoeia"(2010 Edition)records of Ginkgo biloba with blood stasis,andasthma,Tongluozhitong,Huazhuo Jiangzhi,used in blood stasis blocking collaterals,lung deficiency cough,chest pain,hemiplegia,hyperlipidemia.Ginkgo biloba extract in Ginkgo biloba was prepared by alcohol solution extraction and separation and purification of substances,medicinal active ingredients are flavonoids and lactones,in addition,including amino acids,phenolic acids and minerals etc.With the expansion of blood vessels,anti platelet aggregation,anti thrombosis,coagulation,oxidation,lipid regulating effect.Research show that Ginkgo biloba extract can eliminate free radicals,inhibit the cytotoxicity of beta amyloid protein,improve nerve cell degeneration,can obviously improve the symptoms of Alzheimer's disease,delay the aging process.Cognitive,memory,language,orientation,emotional barriers to senile dementia has obvious therapeutic effect and delay.In this paper,Ginkgo biloba extract as a model drug,made the temperature sensitive Ginkgo biloba extract nasal in situ gel.Study on related enzymes activity and malondialdehyde(MDA)content changes,with ginkgo biloba extract temperature sensitive nasal in situ gel treatment for dementia in the plasma,cerebrospinal fluid and brain tissue.Ultraviolet visible spectrophotometry as the detection method,the detection of superoxide dismutase(SOD),catalase(CAT),acetylcholinesterase(AChE)activity and MDA content.Research shows that Ginkgo biloba extract temperature sensitive nasal in situ gel could significantly improve the activity of SOD and CAT,reduce AChE activity and reduce MDA content,It's proved that the preparation has effects on dementia.In vivo experiments,a HPLC-MS/MS analysis method was developed investigate the distribution of Ginkgo biloba extract in plasma.The methodology of HPLC-MS/MS analysis in rat plasma was studied.Results show that the methods of deternining the concentration of Ginkgo biloba extract in plasma by HPLC-MS/MS pharmacokintics is accurate,rapid.This method is applicable for the detection of Ginkgo biloba extract on drug concentration in the plasma,To provide a theoretical basis for the pharmacokinetic study of Ginkgo biloba extract.