Expression And Significance Of TGF-β1, Ki67, UPA In Glioma

Objective:To investigate the transforming growth factor-betal (TGF-beta1), Ki67, urokinase-type plasminogen activator (uPA) expression in glioma tissue and its significance.METHODS:January2007-June2010Kunming Medical University First Affiliated Hospital after surgical resection with pathologically confirmed glioma specimens in48cases, another collection of decompression in patients with traumatic brain injury line10cases of normal brain tissue,immunohistochemical staining (SP) detection of TGF-betal, Ki67expression levels of uPA in different levels of glioma tissue and normal brain tissue, three of the assessment, analysis of expression of the strength and the clinical pathological features.Results:1.1normal brain tissue, I-IV grade gliomas have a TGF-β1-positive invasive, the glioma specimens slice of TGF-betal positive staining in the cytoplasm, there is significant heterogeneity. ANOVA analysis and LSD-T test pairwise comparison, TGF-betal expression in normal brain tissue showed an increasing trend, I-IV grade gliomas, there were significant differences (P=0.000), Spearman rank correlation analysis expression and pathological level was positively correlated (r=0.804, P=0.000). TGF-betal expression in different gender, age, tumor size, tumor location, the difference was not statistically significant, P>0.05.1.2normal brain tissue no Ki67-positive invasive, I-IV grade gliomas were Ki67antigen-positive invasive the glioma specimens slice of Ki67positive staining mainly located in the nucleus, there is significant heterogeneity. ANOVA analysis and LSD-T test pairwise comparison, Ki67expression showed an increasing trend in the I-IV grade gliomas, there were significant differences (P=0.000), Spearman rank correlation analysis showed that the expression of pathological level was positively correlated (r=0.816, P=0.000). Ki67expression in different gender, age, tumor size, tumor location, the difference was not statistically significant, P>0.05.1.3normal brain tissue, Ⅰ-Ⅳ grade gliomas have a UPA-positive invasive, the glioma specimens slice of uPA positive staining in the cytoplasm, there is significant heterogeneity. ANOVA analysis and LSD-T test pairwise comparison, UPA protein positive in normal brain tissue, Ⅰ-Ⅳ grade gliomas showed an increasing trend, there were significant differences (P=0.000), Spearman rank correlation analysis showed that, expression and pathological level was positively correlated (r=0.828, P=0.000). uPA positive expression showed no significant difference in the different gender, age, tumor size, tumor location, P values>0.05.1.4Pearson correlation analysis:TGF-betal, Ki67in glioma was a significant positive correlation (r=0.814, P=0.000); TGF-beta the uPA in glioma was a significant positive correlation (r=0.740, P=0.000); Ki67, the uPA in glioma was positively correlated significantly (r=0.867, P=0.000).Conclusion:TGF-betal, Ki67, UPA high expression with gliomas occur, transformation, proliferation, invasion and metastasis, The three glioma, the role of the development and malignant processes may play a synergy and mutual adjustment.