| Objective:To explore possible mechanisms of comorbid depression type2diabetes, for clinical prevention and treatment of type2diabetes mellitus and depression to provide reference and help. Through molecular level depression before and after the intervention, improve the quality of life in patients with diabetes. This research adopts the combined chain urea with high fat and sugar diet cephalosporins (STZ) intraperitoneal injection production model of type2diabetes, chronic unpredictable mild stress method is used to produce depression model, using high fat and sugar diet combination chain urea with cephalosporins (STZ) intraperitoneal injections and chronic unpredictable mild stress make depression after type2diabetes model method. Observation of diabetes, depression rats and diabetic rats depression rats of behavioural change, hippocampal apoptosis related factor is Bad, the Bcl-xL expression and hippocampal apoptosis morphological changes, agent to discuss the pathogenesis of type2diabetes mellitus and depression might.Method:50healthy adult male SD rats were randomly divided into control group (10), type2diabetes (15), pure depression group (10), type2diabetes mellitus and depression group (15), by intraperitoneal injection of high fat and sugar diet plus chain urine with cephalosporins replication in type2diabetic rats model, the method of chronic unpredictable mild stress model of depression, a combination of depression after type2diabetes model was established, and observe its weight, mainland, diet, blood sugar, etc. Generally, line kuang forced swimming experiment on the behavior of field experiment and test, by HE dyeing observation experiment groups rats hippocampal cells morphological changes, using immunohistochemical method to detect Bad between groups rats hippocampal cells, the expression of Bcl-xL, testing each brain hippocampal apoptosis with TUNEL method, finally use paired t-test; Single factor analysis of variance, paired sample t test and other statistical methods for data analysis and observed the pathological changes of brain tissue.Result:1. During the period of high fat and sugar diet, steady growth in experimental animal groups in terms of weight, after injection of STZ making diabetes model, method of chronic unpredictable mild stress produced during the depression model, diabetes group, the simple depression group, merge depression group rats were losing weight, diabetes group rats drinking water and food intake was significantly increased, the blood sugar significantly increased (P<0.01, P<0.05), urine volume increased, kuang field experiment level scores significantly decreased (P<0.01, P<0.05). Simple depression group rats weight loss significantly (P<0.01, P<0.05), kuang scoring experimental level and vertical score were significantly decreased (P<0.01, P<0.05), forced swimming test measure its swimming time decreased significantly (P<0.01, P<0.05), and motionless time increased significantly (P<0.01, P<0.05). Merge depression group rats weight groups significantly decreased, urine volume increased significantly, a significant rise in blood sugar (P<0.01, P<0.05), kuang scoring the experimental level and vertical scores decreased significantly (P<0.01, P <0.05), forced swimming test determined the time of swimming is obviously less than the rest of the group (P<0.01,P<0.05), and motionless time than the rest of the group increased significantly (P<0.01, P<0.05).2. By HE dyeing observation experiment groups rats hippocampal cells morphological changes:merge depression group rats hippocampal neurons decreased significantly, the cellular level is disorder, partial cell necrosis.3. By immunohistochemical method to detect experiment groups rats hippocampal Bad, the expression of Bcl-xL:diabetes group rats hippocampal cells expressing Bad significantly higher than the control group (P<0.01, P<0.05), the level of expression of Bcl-xL and the control group no significant difference. Simple depression group rats hippocampal cells expressing Bad than the control group increased significantly (P<0.01, P<0.05), the expression of Bcl-xL level than control group significantly decreased (P<0.01,P<0.05), merge depression group Bad expression in rat hippocampus significantly higher than the rest of the group, the Bcl-xL expression level decreased obviously (P<0.01, P<0.05).4. Experimental group by TUNEL method:rat hippocampal cells apoptosis in diabetes group than control group significantly increase the number of rat hippocampal cells apoptosis (P<0.01, P<0.05), pure depression group rats hippocampal apoptosis number has no obvious difference with control group, merge depression group rats hippocampal apoptosis in number than the rest of the experimental group increased significantly (P<0.01, P<0.05).Conclusion:1merge depression group rats weight loss, blood sugar significantly higher, shows that type2diabetes mellitus and depression increased sugar metabolic disorder.2merge depression group rats behavior test, kuang scoring experimental level and vertical scores decreased significantly, forced swimming test rest time significantly increased, significantly reduce the swimming time, shows that type2diabetes mellitus and depression can reduce degree of activities, to explore the ability to drop, desperate degree increase.3group of type2diabetes mellitus and depression mind horse Bad positive cells count results than the rest of the parts of the corresponding results increased significantly, the Bcl-xL positive cell count results than the rest of the parts of the corresponding results significantly reduced. Mind gods by the apoptosis number than the rest of the experimental group increased significantly, type2diabetes mellitus and depression can promote cell apoptosis related proteins Bad rise in promoting apoptosis protein expression, the Bcl-xL inhibiting apoptosis protein expression decreased, promote apoptosis of neurons, which confirmed neuron apoptosis may be the pathogenesis of type2diabetes mellitus and depression a mechanism. |
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