Analysis Of Effective Components In Cortex Magnoliae Officinalis And Evodia Rutaecarpa By Capillary Electrophoresis

Traditional Chinese Medicine (TCM) has been proved to have definite therapeutic effects through clinical application for a long time, and most TCM have slightly or no side effect if administered reasonably. Because of deficiency in objective and strict quality controlling-testing criterion, reliable and systematic scientific experiments to verify its validity and security, TCM is still kept out of the prevailing international medicine market. It is of practical significance to apply this scientific method to modern researches of TCM. As a new advanced method of separation and analysis, Capillary electrophoresis (CE) has been used in the field of analytical chemistry, with such merits as high efficiency, speediness and low cost. By use of organic solvents as separation media, nonaqueous capillary electrophoresis (NACE), which expands the application of CE, has some merits that can't be found in conventional CE. It is provided with original advantages in analyzing materials with poor aqueous solubility. There are mainly two parts in this thesis. In the first part, the development of researches in TCM with CE and NACE was reviewed. In the second part, the content of two kinds of TCM was analyzed by CE and NACE, respectively. The main research contents and conclusions are mentioned as below: 1. A capillary zone electrophoresis method modified with β-cyclodextrin (β-CD) was developed for the purpose of determining the content of honokiol in Cortex Magnoliae Officinalis and Cortex Magnoliae Officinalis-containing preparation. The factors relevant to the running buffer and their effects on separation were investigated. The buffer solution composed of 30 mmol/L sodium borate and 2.4 mmol/L β-CD was found to be the most suitable electrolyte for separation. The second order dissociation constant range of honokiol was estimated. The content range of honokiol in Cortex Magnoliae Officinalis and was 3.42-5.98 mg/g, while the mean content of honokiol in its preparation (Huoxiangzhengqi Oral Liquid) 0.021 mg/mL. A good linear response towards honokiol was found in the range from 0.020 mg/mL to 0.405 mg/mL (R= 0.998, n=5). The recovery ranged between 98.4% and 103.5%. 2. A simple NACE method has been developed for the assay of Rutaecarpine in Evodia rutaecarpa for the first time. Optimum separation of the analytes was obtained using a nonaqueous buffer system with 40 mmol/L ammonium acetate containing 30% acetonitrile and 10% acetate acid. The factors relevant to the running buffer and their effects on separation were discussed. The content of Rutaecarpine in Evodia rutaecarpa was determined. The assay was linear for Rutaecarpine in the concentration range of 0.023-0.46 mg/mL, R=0.9966(n=8). The recovery is 106.3%(n=3).