| Objective:Establish the diabetic rabbit’s radial bone defects model, and repair of bone defects with nano-hydroxyapatite artificial bone. Measure animal blood sugar levels and gross specimen, X-ray plain film, biomechanical testing and hard tissue biopsy tissue morphological observation to observe the process of repairing bone defects in the diabetic state. Discuss the mechanisms of high sugar diabetes status having implications for that the process of nanometer hydroxyapatite bone repair rabbit’s radial bone defects and the creeping substitution of new bone. And come to understand the effects of insulin on nano-hydroxyapatite repairing bone defects of the diabetic rabbits.To provide scientific experimental evidence and theoretical basis for the clinical treatment of bone defects in diabetic patients.Methods:Forty-two6-month-old healthy New Zealand white rabbits,weight2.0-2.5kg,which were randomly divided into30diabetic group and12control group (Group C).In diabetic group,alloxan was intravenously injected by1OOmg/kg body weight to make rabbit diabetes mellitus model, and the equivalent amount of normal saline solution was injected at the same way as normal blood glucose group.14days later,fasting blood glucose was detected, the successful models were selected in the animals with the blood glucose level over16.0mmol/L. The successful models were randomly divided into diabetic group (Group A) and insulin treatment group (Group B)again. The middle of the rabbit radius with the periosteal bone segment resection of1.5cm long to make a standard model of bone defect, then repair bone defects with nano-hydroxyapatite artificial bone. Insulin treatment group received a insulin therapy.At0,48h,3d,1w,2w,4w,6w,8w,10w,12w after operation blood of glucose was measured. X-ray plain film, biomechanical testing and hard tissue biopsy tissue morphological observation taken in the three groups in4th,8th,12th week after the operation. All the data was processed by the SPSS17.0statitical package.Results:1. After alloxan injection rabbits’blood glucose were significantly increase in diabetes group and insulin treatment group. Blood glucose in diabetes group all exceeded16mmol/l.However, there was no significant in the blood glucose level between insulin treatment group and normal group after insulin intervation.2. Antomic observation showed that the differences of grading of artificial bone adhesion were not significant in three groups in the same time (P>0.05). Group A calls growth and the creeping substitution of new bone were slower, there was no significant difference between group B and group C.3. X picture aspect:the X pictures show that the group B and group C were better than group A in osteotylus aspect and the repairing of bone defects aspect. there was no significant difference between group B and group C.4. The results of biomechanical measurement:after implantation of material,the diabetic callus mechanical strength was significantly lower than that in insulin treatment group and normal group in the4th,8th,12th week, there was no significant difference between group B and group C.5. Results of hard tissue slicing-.hard tissue slicing shows that the group B and group C were better than group A in the fibrous connective tissue,bone trabecula and osteoid tissue aspects.And there was no significant difference between group B and group C.According to the score criteria of Lane-Sandhu,shows significant difference(P<0.05) between group A and group C, group A and group C in the same time after operation.Conclusion:1. Rabbits intravenously injected100mg/kg alloxan show low mortality,stable diabetes mellitus model. 2. Nano-HA as the repairing bone defect after implantation of alternative material shows good biocompatibility,degradability and bone conduction,as to repair bone defect for a good alternative to implanted material.3.Diabetes mellitus affect the formation of callus and the repairing of bone defects, insulin can enhance bone formation.4.Diabetes mellitus reduce the biomechanical strength of the bone defect, insulin treatment improve it.5.Diabetes mellitus maybe affect the degradation process of the nano-hydroxy apatite and the creeping substitution of new bone, and then affect Nano-hydroxyapatite ability to repair bone defects. |
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