The Influence Of Extract From Mulberry Leaf On Blood Sugar And Lipid Of Type2Diabetes Model Rat

Objective:To preliminary study on the adjustment mechanism of the extract frommulberry leaf to the blood sugar and lipid of type2diabetes rat models. Method: Afterdetermining the nutrient composition of the main extract of the Mulberry leaf throughrelevant procedure, we randomly divided the experimental samples,72SD male rats, intosix groups: the control group, the model group, also hypoglycemic groups and extract low,medium and high dose groups, according to the random number table. All groups, exceptcontrol group, had been modeled by injecting Streptomyces(STZ) hormone with the doseof45g/kg·bw after having been fed with high fat diet for eight weeks. Then, threeextract-fed groups were fed with high fat foods and different dose of mulberry leaf extract,respectively at0.1ml/100g、0.2ml/100g、0.4ml/100g, once per day. Control group andmodel group got correspondingly fed with ordinary and high fat diet, control and modelgroup got pure water every single day. For the hypoglycemic group were fed with high fatdiet likewise, yet with metformin intervention simultaneously. Result: The mulberryextract contains crude protein22.54g/100g, crude fat6.28g/100g, vitamin B13.57μg/g,vitamin B25.26μg/g and vitamin C26.1μg/g. It also contains High level of calcium andpotassium. The main active substance1-DNJ content is at3.31mg/g, total flavonoids at43.29(mg/g), and rutin at0.24%. Comparing to the result of control group, after fourweeks experiment, the weights of the rats in model group had considerably lowered. Bothtotal cholesterol (TC) and triglyceride (TG) levels of the model group were higher thanthose of control group (p<0.05), and the TG contents in serum extract of high and mediumdose groups and hypoglycemic group were lower than that of model group (p<0.05); TheTC content in serum extract of medium dose group and hypoglycemic group was lowerthan that of model group (p<0.05); It was statistically significant that thedifferences,among the groups, of content of high-density lipoprotein cholesterol (HDL-C)in extract, compared with control group (p<0.05). Before diabetes modeling, the fasting blood glucose (FBG) levels of the rats in each group were not significant difference (P>0.05); Before mulberry leaf extract offering, the FBG levels of the rats in both model andleaf extract fed groups were higher than that of control group(p<0.01). having fed ratswith leaf extract for four weeks, the FBG level of the rats in Hypoglycemic drug groupand each leaf extract group was lower than that of in model group(p<0.05) and self-controlvalue before leaf extract intervention. Fasting insulin and Insulin Sensitivity indexes of themodel group had compared with Hypoglycemic drug group,those of control group,statistical significance (p<0.01), and also the Fins and ISI of Hypoglycemic drug group,thehigh, medium and high dose groups had statistical significance,compared to the result ofblank group (p<0.05). Conclusion: Mulberry leaf extract has some regulative effects ontype Ⅱdiabetic rats, and certain success in controlling the blood sugar of the sick mice;The study also shows that diabetic rats’ mental state is improved, polyuria polyphagiawould be eased, and more shiny skin color appears due to the role of leaf extract.Besides,leaf extract has enduring hypoglycemic effect. If average dose applied, it couldadjust dyslipidemia and lower TC, as well as TG.