The Study Of Biocompatibility Of Hair Follicle Stem Cells And Heterogeneous Bladder Acellular Matrix

Objective:To observation the biocompatibility by construct cells-scaffold compoundthrough the hair follicle stem cells and heterologous bladder acellular matrix in vitro,inorder to provide the experimental basis for study of early HFSCs and heterogeneous BAMcomplex to repair bladder defect. Methods:The HFSCs by using the method of obtainingmicrodissection method, two step enzyme method and differential velocity adherent, andobservation of cell morphology under inverted microscope, identified by the surfacemarkers CK15,β1integrin and CD34. Detection of BAM materials by scanning electronmicroscope and Masson staining.Observation of cell state under the microscope, anddrawn the cell growth curve, histological examination and SEM observation of cells inthe growth status of material surface. Histological examination and scanning electronmicroscopic observation of cells in the growth status of material surface. Results:Thepurified HFSCs colony showed a typical " cobblestone ", the CK15keratin antibodyimmunofluorescence staining was positive, the detection of PE marker flow cytometryshowed high expression of CD34, FITC markers with high expression of β1integrin. Thepreparation of BAM was white and translucent membranous, scanning electronmicroscopic observation of fiber network structure, no residual cells. Masson stainingindicated that BAM collagen structure, no obvious cell residual, cytotoxicity assay showedthat the material had no cytotoxicity. Observation of BAM around HFSCs grew well invitro cell material48h under inverted microscope, cell growth curve of HFSCs-BAMmaterial similar to the control group,7-8days to enter the growth phase, scanning electronmicroscope, adhesion to the HFSCs extension BAM stent surface. Composite cultured invitro for1weeks for histological examination, visible1to3layers of cells spread to thesurface of the scaffold.Observation under inverted microscope, the HFSCs grew wellaround BAMafter48h in vitro, HFSCs-BAM materials on cell7-8days into growth platform.Scanning electron microscopic observation of HFSCs stretching, adhesion toBAM stent surface. Composite cultured7days in vitro for histological examination, wecan see1to3layers of cells spread to the surface of the scaffold. Conclusion:The HFSCsand heterogeneous BAM have good biocompatibility which provide a good experimentsupport for HFSCs to repair the bladder defects disease.