Study On The Metabolites From Marine Fungus100102A Of The East China Sea By Compound Library-Based High Throughput Screening Method

In this study, the active marine fungi from East China Sea were screened with three biological models, of which one active strain named marine fungi100102A was identified. The active secondary metabolites of100102A were separated and studied based on the principle of compound library high-throughput screening method. The conclusion was drawn as follows:(1) Ten marine fungi from the East China Sea were screened by antioxidantive、trypsin inhibitory activity and antitumor activities. And two strains (fungus12010101and08010101) showed strong activities against free radical and trypsase which the inhibition rates were more than90%and45%at the concertraton of2mg·mL-1, respectively. And when the concertation was at100μg·mL-1, the active fungi showed good antitumor activities against PC12、HepG2、U937cells. (2) The active strain of marine fungus100102A which has good antitumor activity against U937cells was identified to Cladosporium sphaerospermum on the basis of its18S rRNA gene sequence.(3) Marine fungus C. sphaerospermum was fermented in50L large scale. The10g crude extract was prepared by solvent extraction. The bioactive molecule guided recognition technology of marine fungus C. sphaerospermum has been established for the first time by the HPLC compound library-based with antioxidantive against DPPH free radical and antitumor activity models.(4) The seven compounds were isolated from crude extracts of marine fungus C. sphaerospermum based on the bioactive molecule guided recognition technology with preparative column under the best conditions of HPLC. The structures of seven compounds were identificated:3-isopentylhexahydropyrrolo [1,2-a] pyrazine-1,4-dione (1)、isopentyloxy-benzene (2)、6-hydroxydecan-5-one (3)、4-butyl-5,6-dihydro-2H-pyran-2-one (4)、(Z)-pentadec-7-en-3-yl propionate (5、bis (2-methylheptyl) phthalate (6)、dibutyl phthalate (7).All isolated compounds were isolated for the first time from marine fungus C. sphaerospermum. The compound7was showed significant inhibitory activity against U937tumor cells with IC50value of198±0.16μg·mL-1Compound2、4、5had slight antitumor activity. In addition, all the compounds showed scavenging activity against DPPH free radical so that compound1-7had different extent of antioxidantive effects.