Research On Epithelial Cells And Cartilage Cells Transplanted By Allogenic Trachea Through Photo-oxidation Reaction Handling Profound Hypothermia Preservation

Research BackgroundTracheal stenosis and defects caused by trauma and tracheal diseases arerelatively common clinically. Successfully conducted tracheal cutting reanastomosisoperation in1881marked the start of tracheal surgery. However, in operativetreatment, if too long trachea is cut, exceeding1/2of that of adults and1/3of that ofchildren, it is necessary to reconstruct trachea. Ideal substitute materials of tracheashall possess the following characteristics:1. sealed lumen structure;2. flexibletexture;3. good biocompatibility;4. low inflammatory reaction;5. low immunity;6.contributing to grow inner wall of tracheal epithelial cells. With depth of medicalstaffs’ research on tracheal reconstruction, there are more and more substitutematerials for tracheal reconstruction, and they become more and more mature.Immunosuppressant is used in rejection reaction of organ transplantation, whichcreates conditions for allogenic trachea transplantation. However, use ofimmunosuppressant may cause relapse of tumor, which may increase tumor relapserate for allogenic trachea transplantation of trachea cancer patients. How to controlimmunological rejection and to reduce antigens of transplants? At present, mainprocessing methods used to reduce antigen of allogenic trachea researched at homeand abroad include radiotherapy of transplants, low temperature preservation, andaccellular etc. Recently, people have found biological materials have such advantagesas non-cytotoxicity, low calcification, weak immunogenicity, and goodbiocompatibility after they are processed through photo-oxidation reaction. In this experiment, the author processed them with the use of photo-oxidation reaction,conducted profound hypothermia preservation without trachea transplantation,observed epithelial cells and cartilage cells of transplanted trachea, evaluatedfeasibility and effectiveness of this photo-oxidation reaction processing technology,and provided a new way method for processing of allogenic trachea transplantation.ObjectiveRabbit trachea through photo-oxidation reaction handling profound hypothermiapreservation, build rabbit model for allogenic trachea transplantation, observeconditions of epithelial cells and cartilage cells, and research feasibility andeffectiveness of this photo-oxidation reaction processing technology.Method36experimental rabbits (male or female, weight:2.5-3kg). They are separatedinto donor group (n=18) and receptor group (n=18) at random. In addition, thereceptor group is separated into profound hypothermia transplantation control group(group A, n=9) and photo-oxidation reaction profound hypothermia transplantationgroup (group B, n=9). Group B is the allosome trachea transplantation groupprocessed with photo-oxidation reaction and stored with liquid nitrogen for six weeks.Group A is the trachea allotransplantation group not processed with photo-oxidationreaction but stored with liquid nitrogen for six weeks. As for group A and group B,operation phase I: bury transplantation trachea in enterocoelia omentum majus ofreceptor rabbits and then conduct revascularization for14days; operation phase II:separate omentum majus through original cut, clear up two ends of transplantationtracheas, which is transplanted to the neck of receptor rabbits. Rabbits were sacrificedand take specimen of transplanted trachea eight weeks after operation phase II.Observe pathologic change microscopically after HE coloration, and calculateepithelium integration and cartilage integration of specimen. ResultsMaterials of group B is light blue after photo-oxidation reaction, with perfectorganization structure. In addition, its flexibility is the same as that of fresh tissue.Organization structure of group A and group B are perfect after liquid nitrogenprofound hypothermia preservation, which don’t have obvious differences whencompared with fresh tissues.During operation phase I, the death rate of rabbits is0%, and living state ofexperimental rabbits fourteen days after experiment; during the operation II, the deathrate of rabbits is0%. One of allogenic trachea of group A and group B respectively isout of shape slightly, after omentum majus embedding, and organization form ofallosome trachea is perfect.Take specimen of transplantation trachea eight weeks after operation phase II.Through observing HE coloration of group A and group B, trachea wall is infiltratedwith lymphocyte and neutrophile granulocyte, with partial thickening endarterium,prominent intra cavity, foam cell aggregation, lymphatic infiltration, andatheromatous plaque. Epithelium integration of group B is obviously higher than thatof group A (p<0.05). Average value of cartilage integration of group B is lower thanthat of group A, but cartilage integration of group A and group B is not differentsignificantly (p>0.05).Conclusions1、Handling profound hypothermia preserved allogenic trachea transplants withthe use of photo-oxidation reaction can boost growth of epithelial cells of transplantedtrachea, which is obviously superior to allogenic trachea transplants preserved withfrozen profound hypothermia independently.2、Allogenic trachea transplants preserved by profound hypothermia with the useof photo-oxidation reaction is not significantly different from allogenic tracheatransplants preserved with frozen profound hypothermia independently inpreservation and regeneration of cartilage cells.