Experimental Research On Rabbit’s Allogenic Trachea Transplantation Through Combining Photo-oxidation Reaction And Profound Hypothermia Treatment

Objective: In this research, the author discussed the feasibility of treating allogenictrachea transplantation with the use of photo-oxidation reaction method after thatrabbit trachea is treated with profound hypothermia, builting rabbit model of allogenictrachea transplantation, and comparing the long-term effects of this method to treatallogenic trachea transplantation with that of simple freezing treatment, so as to findbetter method for treating allogenic trachea and to provide experiment bases for theapplication of allogenic trachea transplantation clinically.Method:1)To cut trachea sections on rabbit’s neck without sterile, and divide theminto two groups at random, namely photo-oxidation freezing group and commonfreezing group. Evaluate influences of different methods on mucous epithelium,cilium, and cartilage cells on the trachea through HE chromoscopy.2)Cut five rings oftrachea sections, and divide them into photo-oxidation freezing group (A) andcommon freezing group (B). Conduct in-situ trachea transplantation respectively afterit is transplanted into the acceptor’s abdominal cavity for about two weeks, andevaluate influences of photo-oxidation reaction on allogenic trachea transplantation inthe aspect of experimental animal’s living state, survival rate, and smoothness oftransplanted tracheas.Results:1) Tracheas stored in two different methods can be complete in tissue andstructure, mucous epithelium and cilium structure and cartilage cell morphology arekept well, and cartilage plate is complete; compared with common freezing, damagescaused by photo-oxidation freezing to cartilage cells are lighter, with higher survivalrate of cartilage cells (P＜0.05). Semi-quantitative grading of mucous epitheliumshows that the former is covered with more complete mucous epithelium and cilium, but there are not statistical differences between them (P＞0.05).2)all experimentalanimals were alive after the orthotopic transplantation,and animals of group Asurvived for more than8weeks.while all experimental animals in group-B (commonfreezing group) die within6weeks. In addition,.they die because of stenosis andobstruction of respiratory tract. It is obvious that smoothness of lumen whose tracheais transplanted with the use of photo-oxidation freezing treatment is better than that ofpositive group.Conclusions:1) Photo-oxidation freezing can gain higher recovery rate of cartilagecells,mucous epithelium and cilium structure and cartilage cell morphology are keptwell,and cartilage plate is complete,whose preservation effects is superior to that ofcommon freezing and damages to tissue and cells are small.2) Photo-oxidationfreezing can preserve tracheas’ structural completeness and lumen smoothes betterafter in-situ transplantation.