Biocompatibility Of Calcium Polyphosphate Fibers/Calcium Phosphate Cement Composite In Vitro Experiment

Objective: Under invitro experimental conditions, evaluate the cell biocompatibilityof Calcium polyphosphate fibers/Calcium phosphate cement composite, meanwhile,discuss the effects to the cell biocompatibility of the composite, whether withdifferent ratio of Calcium polyphosphate fibers and Calcium phosphate cement. Toprovide certain theoretical basis of clinical application this composite.Methods: Calcium polyphosphate fibers and Calcium phosphate cement were madeinto composite modules according to the mass ratio0:10,1:9,2:8,3:7,4:6,5:5. Withthe proportion of1g composite:10ml extraction mediator which is DMEM containing0.1volume fracture fetal bovine serum to prepare of the different proportion ofcomposite leaching solution.Set the negative control group (containing10%fetal bovine serum of DMEM),experiment group(the different proportion of composite material leaching solution)and positive control group (6.4g/l phenol solution).Recovery and subculture MG63cell,cells were cultured with the composite and itsleaching solution respectively:(1) Morphology change of cell was Observed by inverted microscope atdifferent time;(2) The growth and multiplication of cell was detected by MTT method;(3) The apoptosis percentage and cell cycle was detected by flow cytometry.Results:(1)Cells adhered to the composite surface and grew well, the morphology ofcells was normal.(2)Cells were cultured with composite leaching solution together, observe the cells atdifferent time points, cells of the experiment group grew well and rapidly, which was same as the negative control group. They were better than the positive controlgroup obviously, the number of positive control group,cell reduced gradually, themorphology was abnormal, cracked and died at last.(3)MTT method showed that, along with the extension of incubation time, the Avalue of the experimental group and negative group increased gradually, andincreased significantly (P=0.000), in addition to the positive control group. thedifferences of A value between the experimental group and negative group were nostatistical significance (P>0.05) at culturing24h,48h,72h.the differences of A valuebetween the different proportion of the composite material leaching solution groupalso were no statistical significance(P>0.05). The A value of experimental group andnegative group was a little higher than positive control group, but there was nostatistically significant difference(P>0.05), The A value of the positive control groupwas significantly lower than the other two groups(P=0.000)at culturing48h and72h.(4)The cell apoptosis percentage of experimental group and negative group wassimilar and significantly lower than the positive control group after culturing48h.Conclusion: Calcium polyphosphate fibers/Calcium phosphate cement composite haswell cell biocompatibility. Matching different of Composite has no obvious differenceto the osteoblast growth, proliferation and apoptosis effect.