Research On Mechanism Of TFL In Inhibiting Hepatic Fibrosis In Rats

Objective: To study the effects of total flavone from Litchi ChinensisSonn (TFL) on liver cell apoptosis of rats hepatic fibrosis and itsmechanism of action. Methods:40rats randomly divided into fourgroups: blank-control group, model group, TFL high-dose treatmentgroup (200mg/Kg·d) and TFL low-dose treatment group (100mg/Kg·d).Hepatic fibrosis of rats were established by intraperitoneal injection ofdimethylnitrosamine (DMN), then all TFL groups irrigated stomach withdifferent dose of TFL; blank-control group and model group with thesaline water (5ml/kg) as contrast. After the experiment, the rats weresacrificed and liver tissue samples and the serum were collected. HE andMasson staining observed the degree of liver fibrosis; Bcl-2and Baxwere detected with immunohistochemical staining; the serum levels ofalanine aminotransferase (ALT) and aspartate aminotransferase (AST)were determined by automatic biochemical analyzer. Results: Hepaticfibrosis of rats have been established. The expression of Bcl-2and Bax inthe model group was significantly higher than in the normal group (P=0.000, P=0.000); different dose of TFL groups compare to model group increase expression of Bcl-2(P=0.000, P=0.049) and reduce Bax (P=0.000, P=0.018); in TFL high-dose treatment goup of higher expressionof Bcl-2(P=0.019) and lower Bax (P=0.003) than low-dose group. Theseverity of liver fibrosis and the ratio of Bax positive correlation (r=0.930, P=0.000) and Bcl-2negative correlation (r=-0.905, P=0.000).Blank control group and TFL groups the serum levels of ALT(P=0.000,P=0.000, P=0.000) and AST(P=0.000, P=0.000, P=0.000) weresignificantly lower than the model group；in TFL high-dose treatmentgoup the serum levels of ALT (P=0.001) and AST (P=0.000) bothlower than low-dose group. Conclusions: TFL can resistance hepaticfibrosis and reduce the liver cell damage. This role may be by adjustinghigher the expression of Bcl-2and lower Bax, inhibition the liver cellapoptosis to achieve, and have a certain dose-effect relations. Theseverity of liver fibrosis and the ratio of Bcl-2negative correlation andBax positive correlation.