| Objective To investigate the effects of tanshinoneⅡA on the TGF-β1induced proliferationof prostate fibroblasts and expressions of type I and III collagen genes, and thereby toreveal the potential of tanshinone ⅡA in anti-prostate fibrosis.Methods Neonatal rat prostate fibroblasts were harvested by trypsin digestion anddifferential attachment and treated with5ng/mL TGF-β1and different concentrations oftanshinoneⅡA(25,2.5and0.25μg/ml), respectively. PrF proliferation was detected bythiazolyl blue （MTT） colorimetric assay, and flow cytometry was used to detect theeffects of above treatments on the cell cycle of PrF. The expressions of type Ⅰand Ⅲcollagen genes were determined by reverse transcription-polymerase chain reaction(RT-PCR), and the concentrations of oxyproline hydroxyproline were measured to evaluatethe levels of secreted collagen of PrF.Results PrF proliferation, expressions of type Ⅰand Ⅲ collagen genes and concentrationof secreted collagen increased significantly48h after treated by TGF-β1(5ng/ml, P<0.01).TanshinoneⅡA (25,2.5and0.25μg/ml) pretreatment of PrF inhibited PrF proliferation(P<0.05or P<0.01) in a dose-dependent manner, and the flow cytometry revealed that PrFcell cycle was blocked into the G1/G0stage (P<0.05). The results of RT-PCR showed thatthe peak concentration of TanshinoneⅡA (25μg/ml) can downregulate the expressions oftype Ⅰ and Ⅲ collagen genes significantly (P<0.01). From the concentrations ofoxyproline hydroxyproline, we found the significantly negative effect of TanshinoneⅡA onthe secreted collagen of PrF (P<0.05or P<0.01).Conclusion TanshinoneⅡA can inhibit the TGF-β1induced proliferation of prostatefibroblasts and the secretion of collagen, and has the potential of anti-prostate fibrosis. The underlying mechanism was likely related to its inhibition of the proliferation of PrF byblocking PrF cell cycle into the G1/G0stage and downregulating the expressions of typeⅠand Ⅲ collagen genes. |
PDF Full Text Request