Effects Of Granulocye Colony Stimulating Factor On Acute Liver Injure Induced By Lipopolysaccharide In Mice

BackgroundSeveral experimental studies have observed that granulocyte colony stimulatingfactor（G-CSF） may promote hepatic regeneration after hepatectomy in rat or mice andhave a protective role in the treatment of various liver injure, but the issue of whethergranulocyte colony stimulating-factor could have a role in the treatment of chemical liverinjure is still undetermined. Specifcally, few experimental reports have shown that G-CSFcan ameliorate acute liver injure by lipopolysaccharide (LPS) and required furtherexperimental investigation.ObjectiveTo investigate the effect of recombinant human granulocyte colony stimulating factor(rhG-CSF) on acute liver injure induced by LPS in mice.MethodsThe36KM mice were divided randomly into3groups：model group，preventiongroup and normal group. The mice were intraperitoneally administered LPS at10mg/kg（the model group），and then were intraperitoneally injected either rhG-CSF at500μg/kg body weight（the prevention group）or saline（the normal group）at1h before the LPSinjection. The degree of hepatic injury was evaluated at6h and24h after the LPSinjection, and the level of alanine aminotransferase （ALT） and aspartateaminotransferase（AST） in serum was measured by automatic biochemical analyser.Serum TNF-a and IL-10levels were determined by ELISA. Then40KM mice weredivided randomly into2groups：model group and prevention group. The mice wereintraperitoneally administered LPS at30mg/kg （the model group），and then wereintraperitoneally injected either rhG-CSF at500μg/kg body weigh（tthe prevention group）at1h before the LPS injection. The survival rate of the mice was estimated after LPSinjection.ResultsCompared with the model group，the change of liver histology and the levels of ALTand AST were significantly lower in the prevention group（P＜0.05），and the level ofserum IL-10was significantly increased than those in the model group at the6h point（P＜0.05），while there was no signifcant diference at the24h point（P＞0.05）. Theprevention group had serum levels of TNF-a at any time point similar to model group（P＞0.05）. There was no signifcant diference between prevention group and model groupfor the survival rate（P＞0.05）.Conclusion1、Granulocyte colony stimulating-factor can protect the mice from acute liver injureinduced by LPS，its mechanism is related to increasing anti-inflammatory cytokines IL-10production.2、Granulocyte colony stimulating-factor can not enhance the survival rate of micewith endotoxemia.