The Role Of PKC Beta In Effector Cells Of Peripheral Blood And Kidney In Early Stage Murin Graft After Renal Transplantation

Objective: To observe renal tissue and peripheral blood cells infiltration in the grafts,and the role of PKC beta inhibitor played on these effector cells infiltration early after renaltransplantation in mice.Methods: The model of kidney transplantation in mice was established, the mice weredivided into three groups, including allograft control group, allograft PKC beta inhibitortreatment group, and isograft control group. The donor was male BALB/c mouse,and therecipient was female C57BL/6mouse in allogeneic mice renal transplantation model, thedonor and recipient of last set were male and female BALB/c mice respectively. Theallograft PKC beta inhibitor treatment group was given PKC beta inhibitor to the renalrecipient by gavaging2days before and1day after surgery, the same dosage of saline weregiven in the other two groups. After24hours of surgery all of the mice were sacrificed,then taken the peripheral blood and transplant kidney specimens of receptors. Using flowcytometry to detect percentage of lymphocytes in transplant kidney tissues and peripheralblood, as the same time, we detect the positive percentage CD3+, CD4+, CD8+, CD19+and CD4+CD8+of lymphocytes, the CD80+,CD14+,TLR4of monocytes in transplantkidney tissues and peripheral blood.Results: There were significant difference of total lymphocyte and positive percentage ofCD3+cells in transplanted kidney tissues but not in peripheral blood betweenheterogeneous transplantation treatment group, heterogeneous transplantation controlgroup and homogenous transplantation control group. The total lymphocyte infiltration rateand CD3molecules expression levels of treatment group were lower than the control group.There were expression of CD19molecules in peripheral blood but not in transplantedkidney tissues, and there were statistical significance of expression of CD19moleculesbetween the three groups, the treatment group was lower than the control group obviously.There were no significant difference of positive percentage of CD4+, CD8+, CD4+CD8+cells between the three groups, but there were significant difference expression of CD4andCD8molecules between homogenous transplantation control group and heterogeneous transplantation treatment group, the treatment group were higher than the control group.There were no significant difference of positive percentage of CD14+、CD80+、TLR4cellsbetween the three groups.Conclusion: The T and B lymphocytes and mononuclear macrophages participate inimmune rejection and inflammatory reaction process after renal transplantation early. PKCbeta inhibitors can inhibit the activation of T and B lymphocyte, then reduce the immuneresponse. Also can increase the expression level of T lymphocyte subgroup, then improveimmune status and reduce postoperative infection rate of the receptor. PKC beta inhibitorscan inhibit chemotaxis and adhesion ability of mono-nuclear, but can not inhibit theexpression of CD14、CD80、TLR4molecules or the activation and proliferation of inmononuclear macrophages in receptor. In the result, we can know the PKC beta inhibitorscan inhibit the activation of effector cells in receptor after renal transplantation. The doseof clinical application, compatibility with other immunosuppressants and other issues stillneed to further research.