| Objective： Recent studies research found that G0S2and ATGL were the key factors oflipolysis. We investigated the effects of Jueming prescription on the expressions of G0S2andATGL in adipose tissue of diet-induced obese rats, to discuss its effect on lipolysis.Methods：Thirty male SD rats were randomly divided into normal group （7） and modelgroup （23）, feeding standard diet and high fat diet respectively. After4weeks, successfullymodeling obesity rats （21） were randomly divided into three groups: the model group, theJueming prescription-treated group and Semen Cassiae-treated group. The course of thetreatment lasted12weeks, and body weight was measured weekly. The levels of serumglucose and lipid metabolism indicators such as FBG、TG、T-CH、HDL-C、LDL-C weremeasured, and the serum ATGL and INS content were detected using ELISA assay. Thepathological changes of adipose tissue of rats were assessed by the method ofHematoxylin-Eosin stain, and the expression of G0S2and ATGL in adipose tissue wasanalyzed semi-quantitatively with immunohistochemical techniques. The mRNA expressionsof G0S2and ATGL in adipose tissue of SD rats were detected by RT-PCR. And the proteinsexpressions of G0S2and ATGL in adipose tissue were detected by Western Blot.Results: Compared with model group, body weight, weight fat ratio and wet weight ofvisceral fat of rats were significant decreased in Jueming prescription-treated group （P<0.05）.The level of serum TG and LDL-C was markedly increased （P<0.01） and HDL-C increased（P<0.01）, ATGL level distinctly increased （P<0.01）, On the contrary INS level decreased（P<0.01）. The volume of adipocyte was decreased （P<0.01）. The mRNA and proteinexpressions of G0S2in adipose tissue of Jueming prescription-treated rats were markedly decreased, the expressions ofATGL were increased to the contrary （P<0.01）.Conclusion: Jueming prescription could decrease G0S2expression and up-regulate theexpression of ATGL in adipose tissue, which may play the role of weight loss by promotinglipolysis. |
PDF Full Text Request