Preliminary Study On Establishment Of Mouse Model Of Liver Injury By Perfusing Stomach With Dioscorea Bulbifera Decoction

【Objective】To preliminarily explore to establish liver injury mice model byperfusing stomach with hepatic toxicity Chinese medicine dioscorea bulbiferadecoction, and analyse its mechanism through lipid peroxidation damage.【Methods】（1）40Kunming-strain clean mice（weight18-22g）were randomly divided intofour groups, including blank control group, dioscorea bulbifera high dose group,middle dose group and low dose group.10mice in each group, male and female werefifty-fifty. During a6-day period, the mice in dioscorea bulbifera decoction groupswere respectively perfused stomach with dioscorea bulbifera decoction at240g·kg^-1,120g·kg^-1and60g·kg^-1,0.2mL·10g^-1. Simultaneously, the mice in blank control groupwere perfused stomach with equivalent distilled water. Then the levels of ALT, AST inserum were measured, the hepatosomatic index was calculated, and the pathologicalmorphology in liver tissues was observed to find the relative right dioscorea bulbiferadose that could induce liver injury in mice.（2）30Kunming-strain clean mice（weight18-22g）were randomly divided intothree groups, including blank control group,6-day dioscorea bulbifera group and12-day dioscorea bulbifera group.10mice in each group, male and female werefifty-fifty. The mice in dioscorea bulbifera decoction groups were perfused stomachwith the relative right dosage dioscorea bulbifera decoction at0.2mL·10g^-1, andwhich in blank control group were perfused stomach with equivalent distilled water.Then the levels of ALT, AST and TBil in serum were measured, the hepatosomaticindex was calculated, and the pathological morphology in liver tissues was observedto find the relative right time that could induce liver injury in mice. Furthermore, thelevel of MDA, GSH-Px in liver homogenate were measured to preliminarily analyseits mechanism.【Results】（1）Influence on ALT, AST and hepatosomatic index of mice by perfusing stomachwith different dose of dioscorea bulbifera decoction: Compared with blank group, each dose dioscorea bulbifera decoction group has significant effect to the level ofALT, AST in serum and the hepatosomatic index（P<0.05）. The difference of ALT ineach dose dioscorea bulbifera decoction group is obvious, and the level of which ispositively related with the dosage（P<0.05）. The difference of AST between the lowdose group and the middle, high dose group is also Significant（P<0.05）. Thehepatosomatic index has no significant difference in three dioscorea bulbiferadecoction group（P>0.05）.（2）Influence on pathological morphology in liver tissues of mice by perfusingstomach with different dose of dioscorea bulbifera decoction: The mice of low dosegroup have no obvious liver injury. However, the mice of middle and high dose groupappeared to significant liver injury, especially in the high dose group. Semiquantitative analysis of liver cell degeneration indicate that compared with blankgroup, the low dose dioscorea bulbifera decoction has no significant effect to livercell（P>0.005）, while the middle and high dose group is statistically significant（P<0.005）.（3）Influence on ALT, AST, TBil and hepatosomatic index of mice by perfusingstomach with high dose of dioscorea bulbifera decoction for different time: Comparedwith blank group, the level of ALT, AST, TBil in serum and the hepatosomatic indexof mice in6-day group and12-day group is significant increased（P<0.05）. But thereis no statistically significant difference between the two groups（P>0.05）.（4）Influence on pathological morphology in liver tissues of mice by perfusingstomach with high dose of dioscorea bulbifera decoction for different time: The miceof6-day group and12-day group both appeared to significant liver injury. Semiquantitative analysis of liver cell degeneration indicate that compared with blankgroup, it has statistically significant difference（P<0.005）. Similarly, there is nostatistically significant difference between the two groups（P>0.005）.（5）Influence on MDA, GSH-Px in liver homogenate of mice by perfusing stomachwith high dose of dioscorea bulbifera decoction for6days: Compared with blankgroup，the level of MDA, GSH-Px in liver homogenate of mice by perfusing stomachwith high dose of dioscorea bulbifera for6days is not impacted（P>0.05）.【Conclusion】（1）Liver injury mice model can be established by perfusing stomachwith high dose(240g·kg^-1)of dioscorea bulbifera decoction.（2）Free radical lipidperoxidation may not be involved in dioscorea bulbifera decoction induced liverinjury, which still needs to be determined by further experiments.