The Study Of PI3K/Akt Signaling Transduction Pathway Of Rat Vascular Smooth Muscle Cells Proliferation Induced By Apelin

AIM: To observe whether G protein-coupled receptor APJ endogenous ligand apelin-13induce vascular smooth muscle cells (VSMCs) proliferation via PI3K/Akt signalingtransduction pathway, and find new signaling transduction pathway of apelin/APJ.Methods: VSMCs were prepared from thoracic aortas of male Sprague-Dawleyrats by theexplant method. The effect of PI3K inhibitor LY294002and Akt inhibitor1701-1on VSMCsproliferation were measured by MTT assay. Expression of PI3K、pPI3K、pAkt、ERK、pERK1/2and cyclin D1were detected by Western blotting.Results: MTT assay showed that PI3K inhibitor LY294002and Akt inhibitor1701-1significantly inhibited the VSMCs proliferation induced by apelin-13. Western blottinganalysis showed that apelin-13phosphorylated PI3K and Akt in dose and time dependent.PI3K inhibitor LY294002significantly decreased PI3K、Akt、ERK1/2phosphorylation andthe expression of cyclinD1induced by apelin-13; The Akt inhibitor significantly diminishedAkt、ERK1/2phosphorylation and the expression of cyclinD1stimulated by apelin-13.Conclusion: Apelin-13promoted the VSMCs proliferation through PI3K/Akt signalingtransduction pathway.