The Research Of Warming Kidney And Invigorating Spleen Influences On Expression Of Serum IL-8, Colon Tissue NF-κBp65, IL-1β MRNA Of Ulcerative Colitis Model Rats

Objective1. Clarify the spleen kidney Yang deficiency syndrome is the most important syndrome inUC, the method of warming kidney and invigorating spleen is important treatment, and it’son behalf of the Sishen Wan is important Chinese herbal medicine on curing recurrent andrefractory UC.2. Preliminary interpret the mechanism of UC model rats’ intestinal mucosa repair about themethod of warming kidney and invigorating spleen and it’s on behalf of the Sishen Wan.Provide a scientific basis for the method of warming kidney and invigorating spleen curesUC.MethodAdopt the method of “combined disease-and-syndrome”(Fenxieye gavage+glucocorticoid intraperitoneal injection+TNBS/ethanol mixed suspension clyster) preparingspleen kidney Yang deficiency syndrome UC rats model. After the high, medium and lowthree different doses of the Sishen Wan to intervene treatment for21days, at the same timewith SASP treatment as a positive control drug. Then put all the rats to death and dissect.Observe the colon tissue pathological morphology of rats, and detect the serum IL-8level ofrats with ELISA, and NF-κBp65with SP, IL-1β with qRT-PCR expression.Result1. ELISA method detects the rats serum IL-8expression: compared with the blank group,model group rats serum IL-8level increased significantly (P <0.01); after treatment,compared with model group, control group, Sishenwan low, medium and high dose grouprats serum IL-8level reduced significantly (P <0.01).2. SP method detects the rat colonic tissue NF-κBp65expression:(1) microscopically: theblank group NF-κB65positive cells are negative expression, and the model group NF-κB65positive cells which are monocytes infiltrated scatteredly in the submucosa, lymphoidfollicles and outer membrane layer are positive expression in cytoplasm, appear moderate orhigher intensity expression. The rest of the treatment group rats NF-κB65positive cellsappear lower or moderate intensity expression.(2) The NF-κB65positive cells average grey value results show that compared with the blank group, the NF-κB65expression of modelgroup significantly enhanced (P<0.01); after treatment, compared with model group, theNF-κB65expression of control group, Sishenwan medium and high dose groups significantlyreduced (P<0.01).3. RT-qPCR method detects the expression of rat colonic tissue IL-1β mRNA: compared withblank group, the expression of model group IL-1β mRNA significantly enhanced (P<0.01);after treatment, compared with model group, the expression of control group, Sishenwanmedium and high dose group IL-1β mRNAsignificantly reduced (P<0.05).Conclusion1. Sishenwan reduces the UC model rats serum IL-8expression, and suppresses theinflammatory response in the body of the release of active products, thus weakens the body’sinflammatory response.2. Sishenwan reduces the UC model rats colon tissue NF-κBp65expression, and terminatesthe related inflammation gene transcription, and reduces inflammation immune cellproliferation and apoptosis to have the effect of inhibiting the inflammatory reaction.3. Sishenwan reduces the UC model rats colon tissue of the expression of IL-1β, andhindereds the raise of neutrophils to colon and reduces the intestinal mucosal damage.Effectively promote restoration of the intestinal mucosa of rats. Finally, reach the role oftreating diseases.