Expression Of Tumor Suppressor Gene P27Encoding Protein And Cyclin D1in Varioliform Gastritis And Gastric Cancer

ObjectiveVarioliform gastritis is a special type with a characteristic form of chronic gastritis, its etiology and pathogenesis is not clear. In pathological category, there is a certain percentage of varioliform gastritis show intestinal metaplasia and dysplasia. Some studies have prompted that there may be a close relationship between varioliform gastritis and the development of gastric cancer, the molecular mechanism of carcinogenesis is not clear. There is only a few studies about cancer-related genes of varioliform gastritis, we study the expression of p27protein and cyclin Dl in varioliform gastritis and gastric carcinoma to investigate the relationship between varioliform gastritis and gastric cancer, to discuss its possible molecular mechanism of carcinogenesis, and provide more evidence for the prevention and treatment of varioliform gastritis and gastric cancer.MethodsCollect Tissue samples met the research requirements in the gastroscope Center and operating room form January2012to December2012, include40cases of chronic superficial gastritis,40cases of immature varioliform gastritis,40cases of mature varioliform gastritis,40cases of gastric carcinoma. Use the HE staining, analysis the pathological manifestations of chronic superficial gastritis and different type of varioliform gastritis by the microscope. The immunohistochemistry2-step detection system was used to detect the expression of p27protein and cyclin Dl. Urea enzyme method was used to detect the infection of Hp. The data was analysed by SPSS16.0and Excel2003.Result1. The rate of intestinal metaplasia of Immature varioliform gastritis, mature verrucosa gastritis (27.5%,30.0%) were higher than chronic superficial gastritis (7.5%)(P<0.05). There was no significant difference between chronic superficial gastritis, immature varioliform gastritis and mature verrucosa gastritis in the rate of dysplasia (0%,7.5%,12.5%)(P>0.05).2. The positive rates of p27protein expression in chronic superficial gastritis, immature varioliform gastritis, mature verrucosa gastritis, gastritis verrucosa (total), gastric cancer were85.0%,77.5%,52.5%,65.0%,45.0%. The positive rate of p27protein expression in immature varioliform gastritis was higher than mature verrucosa gastritis and gastric cancer (P<0.05), while there was no significant difference between chronic superficial gastritis and immature varioliform gastritis(P>0.05). The positive rate of p27expression in mature verrucosa gastritis was lower than chronic superficial gastritis and immature verrucosa gastritis, while there was no significant difference between mature verrucosa gastritis and gastric cancer (P>0.05).3. The positive rates of cyclin D1expression in chronic superficial gastritis, immature varioliform gastritis, mature verrucosa gastritis, gastritis verrucosa (total), gastric cancer were12.5%,40.0%,42.5%,41.25%,67.5%. The positive rate of cyclin D1expression in immature varioliform gastritis and mature verrucosa gastritis were higher than chronic superficial gastritis (P<0.05), lower than gastric cancer (P<0.05).4.The expression of p27protein and cyclin Dl were not associated with the age and gender of varioliform gastritis patients(P>0.05).5. The Hp infection rates in chronic superficial gastritis, immature varioliform gastritis, mature verrucosa gastritis group were12.5%,35.0%, and40.0%.The positive rate of p27protein expression in Hp positive varioliform gastritis (60.0%) had no difference with Hp negative varioliform gastritis (68.0%) and the positive rate of cyclin D1expression (53.3%,34.0%) was the same result(P>0.05).6.The positive rate of p27protein expression in intestinal metaplasia positive varioliform gastritis group (43.5%) was lower than the intestinal metaplasia negative group(73.6%)(P <0.05).The positive rate of cyclin D1expression in intestinal metaplasia positive varioliform gastritis group (60.9%) was higher than the intestinal metaplasia negative group (33.3%)(P <0.05).7.The positive rate of p27protein expression in dysplasia positive varioliform gastritis (25.0%) was lower than the dysplasia negative varioliform gastritis (69.4%)(P<0.05). There was no significant difference between dysplasia positive and dysplasia negative varioliform gastritis of the positive rate of cyclin Dl expression (62.5%,38.9%)(P>0.05).8.p27expression had no relationship with cyclin D1in varioliform gastritis and gastric cancer(P>0.05).ConclusionsVarioliform gastritis have the potential of cancerization, and mature varioliform gastritis are more likely to have carcinomatous change. p27and cyclin D1may be involved in the process of the occurrence,evolution and cancerization of varioliform gastritis.Varioliform gastritis patients should have active treatment and close follow-up,especially for those patients with intestinal metaplasia or dysplasia.