Expression And Clinical Significance Of14-3-3Protein Family In Endometriotic Cysts Of Ovary

Objective: To investigate the role(s) of14-3-3proteins isoforms played in thedevelopment of endometriotic cysts of ovary, we quantified the expression levels of14-3-3mRNAs and proteins in endometriotic cysts of ovary.Methods: To selecte30histopathologically diagnosed patients with endometrioticcysts of ovary and30controls, then examined the mRNA expressions of all seven14-3-3isoforms in the affected or normal endometriums by RT-PCR. For thosedifferentially expressed isoforms, their protein expressions were further determinedby Western blotting.Results: All of the seven14-3-3isoforms mRNA were detected by RT-PCR both inthe endometriotic cysts of ovary ectopic endometrium and normal endometrium.However, the mRNA expression of14-3-3σ was significantly decreased inendometriotic cysts of ovary ectopic endometrial tissues when compared with thenormal endometrial tissues (P<0.05); the mRNA expressions of14-3-3ε and14-3-3ζwere significantly increased in endometriotic cysts of ovary ectopic endometrialtissues when compared with the normal endometrial tissues (P<0.01); the proteinexpression of14-3-3σ was also significantly decreased in endometriotic cysts ofovary ectopic endometrial tissues (P<0.01); the protein expression levels of14-3-3εand14-3-3ζ were also significantly increased in chocolate of ovary ectopicendometrial tissues (P<0.01).Conclusions：(1) There was a basal mRNA expressions of all the seven14-3-3isoforms both in endometriotic cysts of ovary ectopic endometrium and normalendometrium.(2) The mRNA and protein expressions of14-3-3σ decreased inendometriotic cysts of ovary ectopic endometrial tissues when compared with normalcontrol tissues, the mRNA and protein expressions of14-3-3ε and14-3-3ζ weresignificantly increased. Our results suggested that14-3-3σ, ε, ζ three isoforms of14-3-3proteins may be involved in the development of the endometriotic cysts ofovary.