The Study Of Guanxin Ⅲ Particles On The Inflammatory Factors In Rats With Experimental Acute Myocardial Ischemia

Purpose: Explore Guanxin Ⅲ Particles mechanism of action in the protectionof myocardial ischemia, and lay the foundation for further study, by the studyof Guanxin Ⅲ particles on the the prevention and treatment in rats withexperimental acute myocardial ischemia.Method: The number of120Wistar rats were divided into sham group, modelgroup, Guanxin Ⅲ Particles larger-dose group, middle dose group, low-dose group,through Tongxinluo group, according to the random number table. Using theautomatic biochemical analyzer detect the concentration of the AST，LDH，CK-MB.Using the NB-T staining method and Morphological image processing software todetect myocardial size.Uing the ECG recorded ST-segment before and aftermodeling.Using the ELISA Kit detect the concentration of the IL-6，TNF-α,TXA₂，PGI₂，CRP，TN-I.Using HE staining and electron microscope to see myocardialmorphology.Using Immunohistochemical methods to detect myocardial cell NF-kBexpression.Result: Guanxin Ⅲ Particles can significantly reduce the extent of ECGchanges in rats with acute myocardial ischemia;reduced myocardial infarctsize;reduced the activity of CK-MB，LDH，AST and Tn-I;organization morphologicalchanges with the change of serum creatine kinaseconsistent;can significantlyreduce the content of CRP，IL-6，TNF-α;reduced the concet of TXA₂，elevated thecontent of PGI₂，keeped the physiological balance of PGI₂/TXA₂;inhibit theactivation of NF-kB.Conclusion: Guanxin Ⅲ Particles better protective effect on acutemyocardial ischemic rat myocardium and its mechanism may be related to theinhibition of the vascular endothelial inflammation.