| Objective: Observing their growth and passage through isolating and cultivatingADSCs from rabbit fat tissue. Searching for feasibility of scar mould at rabbit earthrough establishing scar mould at rabbit ear. Explore the new method that ADSCcombinedwith HGF treat hyperplastic scar.Methods:(1)24New Zealand white rabbits (all male), cut the groin area normalsubcutaneous fat tissue under sterile condition. Stem cells obtained by enzyme digestion,cultured in DMEM medium,5×102/ml were inoculated in the culture dish. In theprimary culture of the cells by inverted microscope everyday morphology.(2) makingthe formation of hypertrophic scar in rabbits:10rabbits at ventral ears design1cmdiameter circular excision wound, skin and cartilage and removal of cartilage membrane,hemostatic Oil Sands Covered bandaging, each ear ventral4wound. Wounds formedafter30,50,80and120d, respectively, the proliferative block and the surroundingnormal skin are taken off, fixed by formaldehyde solution, embedded in paraffin, sliced,HE stained, block relative proliferation thickness with a micrometer measuringproliferation at low magnification.(3) the autologous adipose derived stem cells andhepatocyte growth factor used alone and combined application on hypertrophic scar ofrabbit ears. Methods24rabbits were divided into A, B, C, D4groups with6rats ineach group, group A (stem cell and hepatocyte growth factor combined group), group B(hepatocyte growth factor group), group C (adipose stem cells group), group D (salinegroup). Each rabbit was selected2typical hyperplastic scar, hypertrophic scar of rabbitear model after10days,20days and30days in group A local injection of hepatocytegrowth factor and adipose stem cells mixture (1:1), B group of local injection ofhepatocyte growth factor, C group: local injection of adipose stem cells, D group localinjection of saline, thirty-fifth days in the scar, to observe its morphological and biochemical indexes.Results: the extraction cells were fibroblast-like growth, primary cultured cells6-9to60%-80%after fusion, with0.25trypsin digestion after1:2passages. Afterpassage, cell proliferation significantly faster, generally about4D of fusion can bepassaged. Experiments show that, ADSCs activity is good, a strong proliferativecapacity, cell multiplication count cumulative twelfth generations still no significantdownward trend. Scar model after the completion of the twentieth heaven leather afteroperation.35days hyperplasia is most obvious and in this highly lasts about2monthsafter the part begins to soften. A group scar, scar volume were significantly reduced,softer texture, there tends to be flat, not higher than that of normal skin, color is pink,some basic close to normal skin. Group B scar slightly uplift in the surrounding normalskin, the color was purple, group C scar slightly uplift in the surrounding normal skin,color is purple red scar color is pink, group D scar still mostly showed features ofhyperplastic scar, a hill like uplift, higher than normal skin and normal skin2.8~3.2mm. color is bright red, texture hard and tough, but the lesions of the edge does notexceed the scope of operation injury.Conclusion:(1) ASCs in vitro proliferation speed, can obtain a lot of culturedwith differentiation of cells. ASCs has more than50%of the cells can differentiate intoadipocytes mature which is easy to extract, small trauma, get rich source, a hugenumber of features in the human body.(2) in rabbit ear scar model is feasible, the occurrence and development of rabbit earscar with the characteristics of human hypertrophic scar is used for the study of humanhypertrophic scar is reliable.(3) of hepatocyte growth factor and adipose derived stem cells can inhibit the effect ofcombination of scar, hypertrophic scar than using the inhibition of scar alone,hepatocyte growth factor and adipose-derived stem cells stem cell growth thanhepatocyte growth factor on scar inhibited slightly strong. |
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