The Correlation Analysis Of Idiopathic Asthenospermia Patients’ Sperm Peroxiredoxin Ⅰ And Reactive Oxygen Species In Seminal Plasma

Objective:To explore the expression of peroxiredoxin I in patients with idiopathic asthenospermia and its relationship with seminal reactive oxygen species (ROS).Methods:The objects in this study were selected from the infertile male patients (without antioxidant treatment history) in Xiangya Reproductive Center from September2012to December2012.Fresh semen were selected from the experimental group involved26idiopathic asthenospermia patients,and the control group involved15men with fertility history and normal semen analysis parameters. Luminol chemiluminescence method were applied to detect seminal ROS level;Discontinuous density gradient method were used to centrifugalize sperm;Western blotting were utilized to detect the difference of peroxiredoxin I expression between the control group and experimental group.Results:1.The seminal ROS level in experimental groups is significantly higher than in contral group (P<0.01),showed statistically significant negative relationship between seminal ROS level in both groups and sperm activate rate (r=-0.777, P<0.01)2.Compared with the control group,the peroxiredoxin I expression is significantly lower in the experimental group (P<0.01).The sperm peroxiredoxin I content of the two groups was negatively related with seminal ROS level.3.Showed statistically significant positive relationship between peroxiredoxin I content and human sperm progressive motility (r=0.779, P<0.01)Conclusion:1.The decline of peroxiredoxin I expression may be one of the crucial links to cause idiopathic asthenospermia.2.High level ROS is possible one of the main reasons lead to sperm vitality decline in idiopathic asthenospermia patients.