The Experiment Study Of Activate Blood Circulation And Remove Blood Stasis Of Wind Herbs

Purpose:1. Minning the theories and clinical application of wind-expelling herbs treating blood stasis syndrome from the related literature in ancient and modern times for theories and practice basis of wind-expelling herbs activating the blood circulation.2. Studying on the wind-expelling herbs reversing the hepatic fibrosis of lab rats to explore the mechanism of wind-expelling herbs promoting the blood circulation.Methods:1. literature research method:consult the masterpieces in ancient and modern times on wind-expelling herbs invigorating blood circulation to remove blood stasis, and treatments on the diseases of blood stasis with wind-expelling herbs, with the combination of theoretical knowledge of modern clinic practitioners to generalize the characteristics of wind-expelling herbs and minning the theoretical basis of wind-expelling herbs activating the blood circulation.2. experimental methodology:SD rats were classified into5groups:normal contorl, pathological model, colchicine group, xia yu xue tang group and wind herbs (fang feng, qiang huo, baizhi) group. Besides the normal group, others were made into HF models by CCl4subcutaneous injection. Meanwhile, feed each group same amount of normal saline by gavage,8weeks later, model checking succeeded. After12weeks, collected the blood from femoral vain of the rats before killing them to test the serum concentration of HPC-Ⅲ、ColⅣ-C、FN、HA. Microscopic examine the liver histopathological changes of each group by HE coloration. Under uranyl acetate and lead citrate staining, observe the changes of liver cell organelles and nucleus through transmission electron microscope(TEM).Results:1. literature findings:Study on ancient literatures shows that ancient physicians considered most wind herbs have functions like breaking blood stasis and masses, draining blood vessels and meridians, invigorating blood circulation etc. and created numerous practical formulas for clinical use. Furthermore, modern physicians have gained a much deeper and more complete understanding of wind herbs on theoretical level.2. Experimental results:Compare with model control group, wind-expelling herbs group, decoction of discharging blood stagnation (xia yu xue tang) group, colchicines group have certain improvement in the aspects of liver fibrous tissue proliferation, steatosis, hepatocyte edema and chromatin aggregation, mitochondrial swelling, lipid droplets accumulation. The serum concentrations of HPC-Ⅲ, ColⅣ-C, FN, and HA were decreased. Moreover, the improvement of proliferation of liver fibrous tissue, steatosis, liver cell edema, mitochondria swelling, accumulation of lipid droplets in wind-expelling herbs group are much more significant with lower serum HPC-IIIthan that in decoction of discharging blood stagnation group and colchicines group.Conclusions:1.Wind herbs have the function of invigorating the blood circulation, recorded in ancient and modern literatures, which has been widely used in the clinic to treat blood stasis syndrom.2. Wind herbs, like fang feng, bai zhi, qiang huo, can restrain the proliferation of fibrous tissue, fatty degeneration and liver cell edema and liver chromatin aggregation, mitochondrial swelling, accumulation of lipid droplets of hepatic-fibrosised rats, and reduce the serum concentration of ColIV-C, FN, HPC-III especially, which is strongly linked to the major function of wind herbs.3.The mechanism of promoting blood circulation and removing blood stasis of wind herbs may be closely related to their characteristics of pungent, emanative, wandering, promoting and moving. The reason why wind herbs can remove the pathogenic factors of blood stasis syndrome, directly or indirectly disperse blood stasis and treat diseases due to blood stasis is probably related to therapeutic functions of eliminating pathogenic factors, regulating qi to alleviate mental depression, invigorating yang due to the features of pungent and warm, removing dampness to dissolve phlegm, promoting blood circulation by removing blood stasis, reinforcing spleen to elevate yang.