The Essential Oil Of Atractylodes Macrocephala Antitumor Effects And Caspase-3Mechanism、XIAP Mechanism Of Lewis Lung Cancer And Lung Cancer Cell Line A549

Objective:Compared with steam distillation (SD), supercritical fluid extraction (SFE)tumor suppressor role extraction two extraction methods of the essential oil of atractylodesmacrocephala koidz on tumor-bearing mice of Lewis lung cancer, lung cancer cell lineA549, the inhibition rate of extraction method of Rhizoma Atractylodis macrocephalae oilindex optimization and dose, and to explore the the inhibitory mechanism of action, andprovide a reliable basis for the development of natural antitumor drugs.Method：1, in vivo experiment: antitumor effect and its mechanism of essential oil ofAtractylodes macrocephala on tumor-bearing mice of Lewis lung cancer.Establishment of mice model with Lewis lung cancer in mice inoculated with law, thesuccessful model of the mice were randomly divided into SD group, low dose of essentialoil of Atractylodes macrocephala essential oil SD dose group, high dose group of RhizomaAtractylodis Macrocephalae oil SD, Rhizoma Atractylodis Macrocephalae oil SFE lowdose group, middle dose group of Rhizoma Atractylodis Macrocephalae oil SFE, RhizomaAtractylodis Macrocephalae oil high dose group SFE, together with the negative controlgroup, positive control group8group, administered intragastrically for the duration ofAtractylodes macrocephala essential oil emulsion, administration process, themeasurement and calculation of volume of solid tumor in mice; after2weeks ofadministration, based on solid tumor tissue, thymus, spleen, blood, weighed and theinhibition rate of tumor growth, thymus index, spleen index; solid tumor histopathologicaldetection; enzyme linked immunosorbent assay (ELISA) were detected in serum of mice,tumor tissues, the expression of XIAP protein in casepase-3. 2, in vitro experiment: Rhizoma Atractylodis Macrocephalae oil medicated serum onhuman lung cancer cell line A549proliferation inhibition effect and its mechanism.Establishment of human lung cancer A549cell model, cells were divided intonegative control group, positive control group, Rhizoma Atractylodis Macrocephalae oilSD low dose group, middle dose group of Rhizoma Atractylodis Macrocephalae oil SD,Rhizoma Atractylodis Macrocephalae oil high dose group SD, Rhizoma AtractylodisMacrocephalae oil SFE low dose group, middle dose group of Rhizoma AtractylodisMacrocephalae oil SFE, Rhizoma Atractylodis Macrocephalae oil high dose group SFE, atotal of8groups.24hours after serum, MTT assay of human non small cell lung cancerA549cell survival rate, cell morphology was observed under the light microscope, flowcytometry was used to detect A549cell cycle, enzyme linked immunosorbent assay(ELISA) to detect the expression of caspase-3protein in A549cells.Results：1, in vivo:Rhizoma Atractylodis Macrocephalae oil emulsion on tumor-bearing mouse model ofLeiws lung cancer, tumor growth inhibition rate was risingHigh, white atractylodes rhizome oil group SFE inhibitory effect is better than that ofRhizoma Atractylodis Macrocephalae oil group SD; tumor tissue, tumor cells scattereddistribution in tumor tissue, showed extensive necrosis; the expression of Caspase-3inserum, tumor tissues, XIAP expression decreased, compared with the model group hadsignificant difference (P <0.05).The2part, in vitro:Rhizoma Atractylodis Macrocephalae oil containing serum on A549cell line, cellsurvival rate decreased significantly, Rhizoma Atractylodis Macrocephalae oil group SFEinhibitory effect is better than that of rhizoma atractylodis macrocephalae oil group SD;cell cycle arrest in G0/G1phase; caspase-3expression increased in the cells, compared withthe model group had significant difference (P <0.05).Conclusion：1, in vivoRhizoma Atractylodis Macrocephalae oil has inhibitory effect on tumor-bearing miceof Lewis lung cancer, and Rhizoma Atractylodis Macrocephalae oil SFE group was betterthan that of Rhizoma Atractylodis Macrocephalae oil group SD, in which SFE group oflarge dose group the effect is good; Atractylodes macrocephala essential oil can upregulate the expression of Caspase-3in serum, tumor tissues, expression of XIAP in serum, tumortissues.The2part, in vitroRhizoma Atractylodis Macrocephalae oil medicated serum on human non-small celllung cancer cell line A549proliferation, and Rhizoma Atractylodis Macrocephalae oil SFEgroup was better than that of Rhizoma Atractylodis Macrocephalae oil group SD, with thedose of SFE group group effect is good; Atractylodes macrocephala essential oil can blockthe cell cycle at the G0/G1phase, and increase the expression of Caspase-3in the cells.