A Rat Model Of Metabolic Syndrome Induced By High-fructose Diet

Objective:To establish a rat model with metabolic syndrome induced by high-fructose diet. Method:Eight-week-old male Sprague-Dawley rats were divided into two groups:both groups of rats were fed with standard rat chow, however, control group rats (n=15) took normal drinking water and high-fructose group rats (n=33) took15%fructose in drinking water for8weeks. Body weight,24-hour food and drink,12-hour urine, blood pressure, fasting blood glucose and blood biochemical parameter were measured before and after fructose diet. Insulin tolerance test (ITT) and the euglycemic hyperinsulinemic glucose clamp (EHGC) were performed to estimate insulin sensitivity. Results:Rats with high-fructose drinking water showed polydipsia, polyuria and decreased food intake and developed significant hypertension, hyperlipidemia and hyperinsulinemia after8weeks, compared with control rats. High-fructose diet for8weeks did not induce significant obesity and hyperglycemia, but resulted in significant insulin resistance, which was demonstrated in ITT and EHGC. Conclusion:A rat model of metabolic syndrome induced by high-fructose diet was successfully established, which could be beneficial for advanced research of metabolic syndrome and diabetics.